WS-5995 B, an antifungal agent inducing differential gene expression in the conifer pathogen Heterobasidion annosum but not in Heterobasidion abietinum
WS-5995 B, an antifungal agent inducing differential gene expression in the conifer pathogen Heterobasidion annosum but not in Heterobasidion abietinum
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DOI：
10.1007/s00253-009-2254-7
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发表时间：
2009-11-01
影响因子：
5
通讯作者：
Tarkka, Mika T.
中科院分区：
文献类型：
--
作者：
Lehr, Nina A.;Adomas, Aleksandra;Tarkka, Mika T.
菌根化辅助细菌Streptomyces sp. AcH 505抑制挪威云杉根和根腐真菌Heterobasidion annosum 005的感染和定殖，但不抑制同属菌株Heterobasidion abietinum 331，因为H. annosum 005向AcH 505衍生的萘醌抗生素WS-5995 B的转化。两个种的分离株对抗生素敏感性的差异，H。annosum 005和H.在WS-5995 B、结构上相关的软体霉素和不相关的放线菌酮应用后，通过使用宏阵列和定量RT-PCR的比较基因表达分析来研究冷杉素331。用25 μ M WS-5995 B处理2 h导致敏感真菌菌株中肌苷-5 '-单磷酸脱氢酶、磷酸葡萄糖变位酶和GT3基因的表达显著上调，而编码硫氧还蛋白和谷胱甘肽依赖性甲醛脱氢酶的基因的表达下调。在耐受菌株中未检测到差异表达。WS-5995 B在高浓度下的应用实验表明，H. annosum 005和H.冷杉素331对WS-5995 B的反应不同。真菌基因表达水平依赖于WS-5995 B的浓度和施用时间。WS-5995 B-无关放线菌酮引起高度特异性的基因表达模式的变化。我们的研究结果表明相当大的变化，响应细菌代谢产物的针叶树病原体的菌株。
The mycorrhization helper bacterium Streptomyces sp. AcH 505 inhibits Norway spruce root infection and colonisation by the root and butt rot fungus Heterobasidion annosum 005 but not by the congeneric strain Heterobasidion abietinum 331 because of higher sensitivity of H. annosum 005 towards the AcH 505-derived naphthoquinone antibiotic WS-5995 B. Differences in antibiotic sensitivity between two isolates belonging to two species, H. annosum 005 and H. abietinum 331, were investigated by comparative gene expression analysis using macroarrays and quantitative RT-PCR after WS-5995 B, structurally related mollisin and unrelated cycloheximide application. Treatment with 25 A mu M WS-5995 B for 2 h resulted in a significant up-regulation of expression of inosine-5'-monophosphate dehydrogenase, phosphoglucomutase and GTPase genes, while the expression of genes encoding for thioredoxin and glutathione dependent formaldehyde dehydrogenase was down-regulated in the sensitive fungal strain. No differential expression in the tolerant strain was detected. Application of WS-5995 B at higher concentrations over a time course experiment revealed that H. annosum 005 and H. abietinum 331 responded differently to WS-5995 B. The fungal gene expression levels depended on both the concentration of WS-5995 B and the duration of its application. The WS-5995 B-unrelated cycloheximide caused highly specific changes in patterns of gene expression. Our findings indicate considerable variations in response to bacterial metabolites by the isolates of the conifer pathogen.