THE EXPRESSION OF TYROSINE-HYDROXYLASE AND THE TRANSCRIPTION FACTORS CPHOX-2 AND CASH-1 - EVIDENCE FOR DISTINCT INDUCTIVE STEPS IN THE DIFFERENTIATION OF CHICK SYMPATHETIC PRECURSOR CELLS
THE EXPRESSION OF TYROSINE-HYDROXYLASE AND THE TRANSCRIPTION FACTORS CPHOX-2 AND CASH-1 - EVIDENCE FOR DISTINCT INDUCTIVE STEPS IN THE DIFFERENTIATION OF CHICK SYMPATHETIC PRECURSOR CELLS
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DOI：
10.1016/0925-4773(95)00396-i
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发表时间：
1995-07-01
影响因子：
2.6
通讯作者：
ROHRER, H
中科院分区：
文献类型：
--
作者：
ERNSBERGER, U;PATZKE, H;ROHRER, H
交感神经元的肾上腺素能分化由与胚胎环境的复杂相互作用控制。为了对这些相互作用的实验分析提供依据，用免疫组织化学和原位杂交的方法分析了肾上腺素能标志酶酪氨酸羟化酶(TH)在鸡胚胎交感神经节和肾上腺髓质中的表达。同时，用原位杂交技术分析转录因子cPhox-2和Cash-1的发育表达。TH蛋白首先在发育的第三天(阶段19)在初级交感神经链的细胞中被检测到。几小时前(第18期)，原位杂交可检测到TH基因的表达。在同一时间和位置，首次观察到转录因子cPhox-2的mRNA。相反，转录因子Cash-1的mRNA在更早的阶段被检测到，在阶段15，在形成初级交感神经节的主动脉背侧。在胚胎发育后期(35期)，交感神经节和肾上腺嗜铬细胞均维持高水平的TH基因表达。相反，cPhox-2和Cash-1mRNA分别选择性地在嗜铬细胞和交感神经节中减少。结果表明，TH和cPhox-2是交感神经节表达的早期标志物。它们的协同表达指向可能发生在主动脉附近的诱导性事件，并导致肾上腺素能表型的表达。Cash-1被检测到的时间要早得多，这表明它的表达是由另一个单独的事件诱导的。
The adrenergic differentiation of sympathetic neurons is controlled by complex interactions with the embryonic environment. To provide a basis for the experimental analysis of these interactions, the expression of the adrenergic marker enzyme tyrosine hydroxylase (TH) was analyzed by immunohistochemistry and in situ hybridization in sympathetic ganglia and the adrenal medulla of chick embryos. In parallel, the developmental expression of the transcription factors cPhox-2 and Cash-1 was analyzed by in situ hybridization. TH protein was first detectable during the third day of development (stage 19) in cells of the primary sympathetic strands. A few hours earlier (stage 18), TH mRNA could be found by in situ hybridization. At the very same time and location, mRNA for the transcription factor cPhox-2 was first observed. In contrast, mRNA for the transcription factor Cash-1 was detected much earlier, at stage 15, dorsal to the aorta where the primary sympathetic ganglia form. High TH mRNA levels are maintained during later embryonic development (stage 35) in both sympathetic ganglia and adrenal chromaffin cells. In contrast, cPhox-2 and Cash-1 mRNA are selectively reduced in chromaffin cells and sympathetic ganglia, respectively. The results show that TH and cPhox-2 are early markers expressed in sympathetic ganglia. Their coordinated expression points towards an inductive event possibly occurring close to the aorta and leading to the expression of an adrenergic phenotype. Cash-1 is detected significantly earlier, suggesting that its expression is induced by a separate event.