In Vivo Wireless Brain Stimulation via Non-invasive and Targeted Delivery of Magnetoelectric Nanoparticles
In Vivo Wireless Brain Stimulation via Non-invasive and Targeted Delivery of Magnetoelectric Nanoparticles
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DOI：
10.1007/s13311-021-01071-0
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发表时间：
2021-06-15
影响因子：
5.7
通讯作者：
Jin, Xiaoming
中科院分区：
文献类型：
--
作者：
Nguyen, Tyler;Gao, Jianhua;Jin, Xiaoming
对大脑深部结构的无线和精确刺激可能在研究完整的大脑回路和治疗神经系统疾病方面具有重要的应用。在这里，我们报告说，磁电纳米粒子（MENs）可以引导到一个目标的大脑区域，以刺激大脑活动的磁场。我们证明了纳米颗粒在离体皮质切片中可靠地引起快速神经元反应的能力。在荧光标记的MENs静脉注射并通过施加磁场梯度递送到目标脑区域后，施加到小鼠头部的低强度（350-450 Oe）的磁场可靠地诱发皮质活动，如通过钙信号的双光子和介观成像以及刺激后c-Fos表达细胞数量增加所揭示的。无论是大脑交付的MENs，也没有磁刺激引起星形胶质细胞和小胶质细胞的显着增加。因此，MENs可以实现非侵入性和非接触式脑深部刺激，而无需基因操作。
Wireless and precise stimulation of deep brain structures could have important applications to study intact brain circuits and treat neurological disorders. Herein, we report that magnetoelectric nanoparticles (MENs) can be guided to a targeted brain region to stimulate brain activity with a magnetic field. We demonstrated the nanoparticles' capability to reliably evoke fast neuronal responses in cortical slices ex vivo. After fluorescently labeled MENs were intravenously injected and delivered to a targeted brain region by applying a magnetic field gradient, a magnetic field of low intensity (350-450 Oe) applied to the mouse head reliably evoked cortical activities, as revealed by two-photon and mesoscopic imaging of calcium signals and by an increased number of c-Fos expressing cells after stimulation. Neither brain delivery of MENs nor the magnetic stimulation caused significant increases in astrocytes and microglia. Thus, MENs could enable a non-invasive and contactless deep brain stimulation without the need of genetic manipulation.