Antibodies to Synthetic Peptide Immunogens as Probes for Virus Protein Expression and Function
Antibodies to Synthetic Peptide Immunogens as Probes for Virus Protein Expression and Function
复制标题
合成肽免疫原抗体作为病毒蛋白表达和功能的探针
DOI：
--
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
R. Lerner
中科院分区：
文献类型：
--
作者：
T. Shinnick;J. Sutcliffe;R. Lerner
总体而言，病毒遗传结构的研究方向是从表型追踪到基因型。目前可用的重组DNA和核酸测序技术在一定程度上扭转了这一过程，我们开始发现自己在寻找表型时使用基因型，特别是当研究工作从有关已知病毒和病毒蛋白的问题转向有关最近分离的或很少研究的病毒的问题时。事实上，从病毒的核苷酸序列中，人们可以了解有关病毒基因组的遗传结构、其编码能力及其进化历史的大量信息。然而，我们需要的是一种将核苷酸序列中可用的丰富遗传信息充分利用到生物实验中的方法。此外，由于蛋白质是负责病毒大部分生物学重要活性和特性的物质，因此人们面临着一项不那么简单的任务，即鉴定病毒基因的蛋白质产物并生成探针来研究蛋白质表达、加工和生物活性。换句话说，如何识别仅从一串核苷酸序列推断其存在的蛋白质？
In general, the direction of research into the genetic structure of viruses has been that of tracing phenotype to genotype. The currently available recombinant DNA and nucleic acid sequencing techniques have somewhat reversed this process and we are beginning to find ourselves with genotypes in search of phenotypes, especially as research efforts move from questions about known viruses and viral proteins to questions about recently isolated or little-studied viruses. Indeed, from the nucleotide sequence of a virus, one can learn a great deal about the genetic structure of the viral genome, its coding capacity and its evolutionary history. What is needed, however, is a way to parlay the wealth of genetic information available in the nucleotide sequence into biological experiments. Furthermore, since proteins are the agents responsible for most of the biologically important activities and properties of viruses, one is faced with the not-so-trivial task of identifying the protein products of the viral genes and generating probes to study protein expression, processing, and biological activities. In other words, how does one identify a protein whose existence is merely inferred from the sequence of a string of nucleotides?