LIPOCALIN-PROSTAGLANDIN D SYNTHASE IS A CRITICAL BENEFICIAL FACTOR IN TRANSIENT AND PERMANENT FOCAL CEREBRAL ISCHEMIA
LIPOCALIN-PROSTAGLANDIN D SYNTHASE IS A CRITICAL BENEFICIAL FACTOR IN TRANSIENT AND PERMANENT FOCAL CEREBRAL ISCHEMIA
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DOI：
10.1016/j.neuroscience.2009.02.039
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发表时间：
2009-04-21
期刊：
影响因子：
3.3
通讯作者：
Dore, S.
中科院分区：
文献类型：
--
作者：
Saleem, S.;Shah, Z. A.;Dore, S.
前列腺素D-2 （PGD(2)）是大脑中最丰富的前列腺素。它是花生四烯酸的代谢物，由前列腺素D-2合成酶（PGDS）通过环氧化酶途径合成。目前已鉴定出两种不同类型的前列腺素D合成酶：造血前列腺素D合成酶（H-PGDS）和脂钙素型前列腺素D合成酶（L-PGDS）。由于对L-PGDS在中枢神经系统中的作用知之甚少，我们在这里研究了L-PGDS敲除和野生型（WT）小鼠在两种不同的脑缺血模型，即短暂性大脑中动脉（MCA）闭塞（tMCAO）和永久性大脑中动脉远端闭塞（pMCAO）后的结果。在tMCAO模型中，用单丝闭塞MCA 90 min，然后再灌注4 d。在pMCAO模型中，MCA远端永久闭塞，7天后处死小鼠。校正后的梗死体积百分比和神经学评分分别在4天和7天后测定。经tMCAO后，L-PGDS敲除小鼠的梗死面积和脑水肿明显大于WT小鼠(P
Prostaglandin D-2 (PGD(2)) is the most abundant prostaglandin produced in the brain. It is a metabolite of arachidonic acid and synthesized by prostaglandin D-2 synthases (PGDS) via the cyclooxygenase pathway. Two distinct types of PGDS have been identified: hematopoietic prostaglandin D synthase (H-PGDS) and lipocalin-type prostaglandin D synthase (L-PGDS). Because relatively little is known about the role of L-PGDS in the CNS, here we examined the outcomes in L-PGDS knockout and wild-type (WT) mice after two different cerebral ischemia models, transient middle cerebral artery (MCA) occlusion (tMCAO) and permanent distal middle cerebral artery occlusion (pMCAO). In the tMCAO model, the MCA was occluded with a monofilament for 90 min and then reperfused for 4 days. In the pMCAO model, the distal part of the MCA was permanently occluded and the mice were sacrificed after 7 days. Percent corrected infarct volume and neurological score were determined after 4 and 7 days, respectively. L-PGDS knockout mice had significantly greater infarct volume and brain edema than did WT mice after tMCAO (P