Comparative study of the ability of mesenchymal stem cells derived from bone marrow, periosteum, and adipose tissue in treatment of partial growth arrest in rabbit
Comparative study of the ability of mesenchymal stem cells derived from bone marrow, periosteum, and adipose tissue in treatment of partial growth arrest in rabbit
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DOI：
10.1089/ten.2005.11.904
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发表时间：
2005-05-01
影响因子：
--
通讯作者：
Lee, EH
中科院分区：
文献类型：
--
作者：
Hui, JHP;Li, L;Lee, EH
本研究评估了从不同来源（骨髓、骨膜或脂肪）分离的 MSC 治疗未成熟（6 周龄）新西兰白兔部分生长停滞的能力。这些新西兰白兔的胫骨近端骺板内侧半部高达 50% 被切除。三周后，切除骨桥，将含有和不含间充质干细胞的纤维蛋白胶转移到不同兔子的骨骺缺损处。对侧胫骨不进行手术，作为自我控制。四组兔子参与了这项研究。每组分别注射骨髓来源的 MSC（I 组）、骨膜来源的 MSC（II 组）、脂肪来源的 MSC（III 组）和单独的纤维蛋白胶（对照组，IV 组）。术后8周和16周处死兔子。随后进行临床、放射学和组织学分析。在原代培养的第 4、7 和 11 天，三种 MSC 的增殖率相似。然而，来自骨髓和骨膜的间充质干细胞似乎比来自脂肪的间充质干细胞更均匀。所有 MSC 均在体外表现出软骨形成和成骨分化潜力。第 I 组和第 II 组的胫骨在 16 周时显示出内翻角度的显着校正。然而，与 I 组相比，III 组的内翻角度仍然非常明显（p < 0.05）。与对照组相比，第一组、第二组和第三组中手术胫骨和正常胫骨之间的长度差异得到显着纠正（p < 0.01）。总之，骨髓和骨膜比脂肪产生更多同质间充质干细胞，从而更好地纠正兔子的骨骺停滞。间充质干细胞的来源本身可能会影响生长停滞治疗的成功。
This study evaluates the ability of MSCs isolated from different origins - bone marrow, periosteum, or fat - to treat partial growth arrest in immature (6-week-old) New Zealand White rabbits. Up to 50% of the medial half of the proximal physis of the tibia was excised in these New Zealand White rabbits. Three weeks later, the bony bridge was excised, and fibrin glue with and without MSCs were transferred into the physeal defect of different rabbits. Contralateral tibias, without undergoing operation, served as self- control. Four groups of rabbits were involved in the study. Each group was injected separately with bone marrow-derived MSCs (group I), periosteum-derived MSCs (group II), fat-derived MSCs (group III), and fibrin glue alone (control, group IV). The rabbits were killed 8 and 16 weeks postoperatively. Clinical, radiological, and histological analyses were subsequently performed. Similar proliferative rates for three MSCs were demonstrated on days 4, 7, and 11 of primary culture. However, MSCs derived from bone- marrow and periosteum appeared to be more homogeneous than that from fat. All MSCs demonstrated chondrogenic and osteogenic differentiation potentials in vitro. The tibias in groups I and II showed significant correction of varus angulation at 16 weeks. However, the varus angulation in group III remained significantly obvious when compared with group I (p < 0.05). The length discrepancies between operated and normal tibiae in groups I, II, and III were significantly corrected compared with control (p < 0.01). In conclusion, bone- marrow and periosteum yielded more homogenous MSCs than fat, providing better correction of physeal arrest in rabbits. The source of MSCs itself could influence the success in the treatment of growth arrest.