Biomimetic modification of chitosan with covalently grafted lactose and blended heparin for improvement of in vitro cellular interaction
Biomimetic modification of chitosan with covalently grafted lactose and blended heparin for improvement of in vitro cellular interaction
复制标题
DOI：
10.1002/pat.962
复制
发表时间：
2008
影响因子：
3.4
通讯作者：
H. Tan;L. Lao;Jindan Wu;Yihong Gong;Changyou Gao
中科院分区：
文献类型：
--
作者：
H. Tan;L. Lao;Jindan Wu;Yihong Gong;Changyou Gao
制备了乳糖和肝素修饰的壳聚糖膜，并与壳聚糖、壳聚糖-g-肝素和壳聚糖-g-乳糖膜的物理和生物学性能进行了比较。原子力显微镜(AFM)测试表明，这些薄膜在干态下都比较平整，粗糙度小于20 nm。壳聚糖-g-乳糖/肝素和壳聚糖-g-乳糖膜的溶胀率和失重率最高，壳聚糖和壳聚糖-g-肝素膜最低。壳聚糖-g-乳糖/肝素复合膜对软骨细胞的贴壁、增殖、存活和糖胺聚糖(GAG)分泌的诱导作用明显强于壳聚糖、壳聚糖-肝素和壳聚糖-g-乳糖复合膜。在壳聚糖-g-乳糖/肝素和壳聚糖-g-乳糖膜上可见软骨细胞聚集和结节，仍保持了活性代谢能力。这些结果表明，乳糖修饰和肝素掺杂的壳聚糖膜可以同时增强细胞-生物材料的相互作用。所制备的壳聚糖-g-乳糖/肝素材料具有更高的生物活性，有望成为软骨形成的支架材料。版权所有©2007 John Wiley&Sons，Ltd.
Lactose- and heparin-modified chitosan films were prepared and their physical and biological properties were compared with chitosan, chitosan-g-heparin, and chitosan-g-lactose films. Atomic force microscopy (AFM) measurement showed that all these films in the dry state were rather flat with a roughness smaller than 20 nm. While the chitosan-g-lactose/heparin and chitosan-g-lactose films have the highest swelling and weight loss ratios, the chitosan and chitosan-g-heparin films have the lowest. The chitosan-g-lactose/heparin film showed stronger ability to induce chondrocyte attachment, proliferation, viability, and glycosaminoglycan (GAG) secretion than that of the chitosan, chitosan-g-heparin, and chitosan-g-lactose films. Chondrocyte aggregates and nodules were observed on the chitosan-g-lactose/heparin and chitosan-g-lactose films, which still preserved viable metabolic ability. These results show that the lactose-modified and heparin-incorporated chitosan film can enhance the cell–biomaterial interaction synchronously. The resulting chitosan-g-lactose/heparin material is more bioactive that might be applicable as promising scaffold for chondrogenesis. Copyright © 2007 John Wiley & Sons, Ltd.