Normalization of optical fluence distribution for three-dimensional functional optoacoustic tomography of the breast.
Normalization of optical fluence distribution for three-dimensional functional optoacoustic tomography of the breast.
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DOI：
10.1117/1.jbo.27.3.036001
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发表时间：
2022-03
影响因子：
3.5
通讯作者：
Oraevsky AA
中科院分区：
文献类型：
--
作者：
Park S;Brooks FJ;Villa U;Su R;Anastasio MA;Oraevsky AA
在三维（3D）功能性光声断层扫描（OAT）中，波长相关的光学衰减和不均匀的入射光通量限制了成像深度和视场，并可能阻碍对功能量（例如血管血氧合）的准确估计。这些限制阻碍了大对象（诸如人类女性乳房）的OAT。我们的目标是开发一种测量数据驱动的方法，用于光通量分布的归一化，并研究血管系统的可检测性和估计血管血氧的准确性。所提出的方法是基于合理的假设乳房解剖和光学特性。基于OAT系统中的照明几何形状来估计非均匀入射光通量，并且使用Beer-Lambert定律来近似深度依赖的光学衰减。数值研究表明，所提出的方法显着提高血管的可检测性和提高估计精度的血管血氧从多波长OAT测量，相比直接应用光谱线性解混没有光学通量补偿。实验结果表明，所提出的方法揭示了以前不可见的结构，在区域深超过15毫米和/或附近的胸壁。所提出的方法提供了一个简单的和计算成本低廉的近似波长相关的有效光学衰减，因此，能够减轻功能性3D OAT成像中的光谱着色效应。
In three-dimensional (3D) functional optoacoustic tomography (OAT), wavelength-dependent optical attenuation and nonuniform incident optical fluence limit imaging depth and field of view and can hinder accurate estimation of functional quantities, such as the vascular blood oxygenation. These limitations hinder OAT of large objects, such as a human female breast. We aim to develop a measurement-data-driven method for normalization of the optical fluence distribution and to investigate blood vasculature detectability and accuracy for estimating vascular blood oxygenation. The proposed method is based on reasonable assumptions regarding breast anatomy and optical properties. The nonuniform incident optical fluence is estimated based on the illumination geometry in the OAT system, and the depth-dependent optical attenuation is approximated using Beer–Lambert law. Numerical studies demonstrated that the proposed method significantly enhanced blood vessel detectability and improved estimation accuracy of the vascular blood oxygenation from multiwavelength OAT measurements, compared with direct application of spectral linear unmixing without optical fluence compensation. Experimental results showed that the proposed method revealed previously invisible structures in regions deeper than 15 mm and/or near the chest wall. The proposed method provides a straightforward and computationally inexpensive approximation of wavelength-dependent effective optical attenuation and, thus, enables mitigation of the spectral coloring effect in functional 3D OAT imaging.