Synthesis and characterization of poly (N-isopropylacrylamide)-g-carboxymethyl chitosan copolymer-based doxorubicin-loaded polymeric nanoparticles for thermoresponsive drug release
Synthesis and characterization of poly (N-isopropylacrylamide)-g-carboxymethyl chitosan copolymer-based doxorubicin-loaded polymeric nanoparticles for thermoresponsive drug release
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DOI：
10.1007/s00396-015-3804-4
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发表时间：
2016-03-01
影响因子：
2.4
通讯作者：
Kandasamy, Ruckmani
中科院分区：
文献类型：
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作者：
Antoniraj, M. Gover;Kumar, C. Senthil;Kandasamy, Ruckmani
将氨基端PNIPAm与o -羧甲基壳聚糖通过接枝法制备PNIPAm-壳聚糖热响应接枝共聚物（PNIPAm-g- ocmc）。FTIR和质子核磁共振的结果证实了共聚物的合成。此外，XRD分析还揭示了高分子材料的共轭反应。采用凝胶法制备了负载阿霉素的聚合物纳米颗粒（D-PNPs）。透射电镜形貌显示D-PNPs呈球形。粒径测量研究表明了D-PNPs在不同温度下的热响应特性。浊度法预测D-PNPs的较低临界溶液温度（LCST）为38℃。在三种不同温度下的释放研究表明，D-PNPs的热响应性多柔比星释放。阿霉素在25℃时释放较慢，在37℃时释放适度，在42℃时由于d - pnp结构的调整释放较快。基于透射率测量、粒径观察和释放研究等结果，我们宣布D-PNPs表现出温度响应行为，这可以使它们适合热响应性药物递送。
Thermoresponsive graft copolymer (PNIPAm-g-OCMC) with PNIPAm and chitosan was prepared by conjugation of amine-terminated PNIPAm with O-carboxymethylchitosan through graft-onto method. The results of FTIR and proton NMR confirmed the synthesis of a copolymer. Furthermore, XRD analysis revealed conjugation reaction of polymeric materials. Doxorubicin-loaded polymeric nanoparticles (D-PNPs) were fabricated using copolymer by gelation method. Morphology illustration through TEM showed the spherical shape of D-PNPs. Particles size measurement study indicates thermoresponsive properties of D-PNPs at various temperatures. Lower critical solution temperature (LCST) of D-PNPs was obtained at 38 A degrees C predicted by turbidimetry analysis. Release studies at three different temperatures notified that thermoresponsive doxorubicin release of D-PNPs. The release of doxorubicin was slower at 25 A degrees C, moderately released at 37 A degrees C, and faster released at 42 A degrees C due to adjustment of the framework of the D-PNPs. Based on the results such as transmittance measurement, particle size observation, and release studies, we declare that the D-PNPs exhibit temperature-responsive behavior, which could make them appropriate for a thermoresponsive drug delivery.