Identification and characterization of chitinolytic bacteria isolated from a freshwater lake.
Identification and characterization of chitinolytic bacteria isolated from a freshwater lake.
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从淡水湖中分离的几丁质分解细菌的鉴定和表征。
DOI：
10.1080/09168451.2017.1422969
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Suzuki K.
中科院分区：
文献类型：
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作者：
Tran DM;Sugimoto H;Nguyen DA;Watanabe T;Suzuki K.
为了开发一种新型的生物防治剂，我们专注于细菌，其特征在于两个几丁质酶活性和生物膜的发展。从日本新泻酒田沙丘湖的沉积物和浸于水中的几丁质薄片中分离出几丁质分解细菌。从5100多株分离菌株中筛选出31株进行几丁质酶活性和生物被膜形成的测定。根据16S rRNA基因序列对分离株进行系统发育分析，结果表明，分离株主要属于气单胞菌科，其次是类杆菌科、肠杆菌科和奈瑟菌科。筛选出的4株菌的几丁质酶比活力均高于参比菌株。Andreprevotia产生的几丁质酶的分子大小大于典型的细菌几丁质酶。4株菌的几丁质酶透析培养上清均能抑制里氏木霉菌丝生长。这些结果表明，这四个菌株是良好的候选生防剂。
To develop a novel type of biocontrol agent, we focus on bacteria that are characterized by both chitinase activity and biofilm development. Chitinolytic bacteria were isolated from sediments and chitin flakes immersed in the water of a sand dune lake, Sakata, in Niigata, Japan. Thirty-one isolates from more than 5100 isolated strains were examined chitinase activity and biofilm formation. Phylogenetic analysis of these isolates based on the 16S rRNA gene sequences revealed that most isolates belonged to the familyAeromonadaceae, followed byPaenibacillaceae,Enterobacteriaceae, andNeisseriaceae. The specific activity of chitinase of four selected strains was higher than that of a reference strain. The molecular size of one chitinase produced byAndreprevotiawas greater than that of typical bacterial chitinases. The dialyzed culture supernatant containing chitinases of the four strains suppressed hyphal growth ofTrichoderma reesei. These results indicate that these four strains are good candidates for biocontrol agents.