Total internal reflection fluorescence microscopy imaging-guided confocal single-molecule fluorescence spectroscopy.
Total internal reflection fluorescence microscopy imaging-guided confocal single-molecule fluorescence spectroscopy.
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DOI：
10.1063/1.3677334
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发表时间：
2012-01
期刊：
影响因子：
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通讯作者：
Desheng Zheng;Leonora Kaldaras;H. Peter Lu
中科院分区：
文献类型：
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作者：
Desheng Zheng;Leonora Kaldaras;H. Peter Lu
我们已经开发了一个集成的光谱系统相结合的全内反射荧光显微镜成像与共焦单分子荧光光谱的二维界面。这种光谱方法能够同时对多个分子进行采样，并对感兴趣的单个分子进行原位共聚焦荧光动力学分析。我们已经证明了荧光微球的校准，并进行了单分子光谱测量。这种集成的单分子光谱学在生物和化学系统界面的单分子动力学研究中是强大的。
We have developed an integrated spectroscopy system combining total internal reflection fluorescence microscopy imaging with confocal single-molecule fluorescence spectroscopy for two-dimensional interfaces. This spectroscopy approach is capable of both multiple molecules simultaneously sampling and in situ confocal fluorescence dynamics analyses of individual molecules of interest. We have demonstrated the calibration with fluorescent microspheres, and carried out single-molecule spectroscopy measurements. This integrated single-molecule spectroscopy is powerful in studies of single molecule dynamics at interfaces of biological and chemical systems.