Synergistic and Antagonistic Interactions among the Particulate Matter Components in Generating Reactive Oxygen Species Based on the Dithiothreitol Assay.
Synergistic and Antagonistic Interactions among the Particulate Matter Components in Generating Reactive Oxygen Species Based on the Dithiothreitol Assay.
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DOI：
10.1021/acs.est.7b04261
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发表时间：
2018-01
影响因子：
11.4
通讯作者：
Haoran Yu;Jinlai Wei;Yilan Cheng;Kiran Subedi;V. Verma
中科院分区：
文献类型：
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作者：
Haoran Yu;Jinlai Wei;Yilan Cheng;Kiran Subedi;V. Verma
我们评估了颗粒物（PM）组分之间的相互作用，在产生活性氧（ROS）的基础上二硫苏糖醇（DTT）测定。我们从已知氧化还原活性物质的标准溶液开始，即，醌类（9，10-菲醌、1，2-萘醌、1，4-萘醌和5-羟基-1，4-萘醌）和金属[Fe（II）、Mn（II）和Cu（II）]。在DTT测定中测量DTT消耗和羟基自由基（·OH）产生。Fe与醌类化合物的相互作用对DTT的消耗具有相加作用，但对·OH的产生具有强烈的协同作用。Cu与醌类在DTT消耗和·OH产生方面均表现出拮抗作用。Mn与醌类化合物在DTT氧化中存在协同作用，而在·OH生成中存在拮抗作用。这些金属（Fe，Mn和Cu）与环境类腐殖物质（HULIS）的相互作用的性质类似于与醌类的相互作用，尽管相互作用的强度在DTT消耗中比·OH产生弱。最后，我们证明，DTT的环境PM的消费能力可以很好地解释由HULIS，三种过渡金属（铁，锰，铜），和它们的相互作用，但·OH的产生涉及的贡献（约50%）从其他化合物（脂肪族物种或金属以外的铁，锰，铜）存在于亲水PM馏分。该研究强调了需要考虑有机化合物和金属之间的相互作用，同时分配PM氧化电位中化学成分的相对贡献。
We assessed the interactions among the particulate matter (PM) components in generating the reactive oxygen species (ROS) based on a dithiothreitol (DTT) assay. We started with the standard solutions of known redox-active substances, i.e., quinones (9,10-phenanthraquinone, 1,2-naphthoquinone, 1,4-naphthoquinone, and 5-hydroxy-1,4-naphthoquinone) and metals [Fe (II), Mn (II), and Cu (II)]. Both DTT consumption and hydroxyl radical (·OH) generation were measured in the DTT assay. The interactions of Fe were additive with quinones in DTT consumption but strongly synergistic in ·OH generation. Cu showed antagonistic interactions with quinones in both DTT consumption and ·OH generation. Mn interacted synergistically with quinones in DTT oxidation but antagonistically in ·OH generation. The nature of the interactions of these metals (Fe, Mn, and Cu) with ambient humic-like substances (HULIS) resembled that with quinones, although the intensity of interactions were weaker in DTT consumption than ·OH generation. Finally, we demonstrated that the DTT consumption capability of ambient PM can be well explained by HULIS, three transition metals (Fe, Mn, and Cu), and their interactions, but ·OH generation involves a contribution (∼50%) from additional compounds (aliphatic species or metals other than Fe, Mn, and Cu) present in the hydrophilic PM fraction. The study highlights the need to account for the interactions between organic compounds and metals, while apportioning the relative contributions of chemical components in the PM oxidative potential.