Ghrelin and des-acyl ghrelin: Two major forms of rat ghrelin peptide in gastrointestinal tissue
Ghrelin and des-acyl ghrelin: Two major forms of rat ghrelin peptide in gastrointestinal tissue
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DOI：
10.1006/bbrc.2000.4039
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发表时间：
2000-12-29
影响因子：
3.1
通讯作者：
Kangawa, K
中科院分区：
文献类型：
--
作者：
Hosoda, H;Kojima, M;Kangawa, K
Ghrelin是一种从胃中提纯的新多肽，是生长激素促分泌剂受体的内源性配体，具有很强的生长激素释放活性。Ghrelin的Ser3残基被正辛酸修饰，这是激素活动所必需的修饰。我们建立了两种Ghrelin特异的放射免疫分析方法；一种识别Ghrelin的辛酰修饰部分，另一种识别Ghrelin的C末端部分，使用这些放射免疫分析系统，我们发现存在两种主要的分子形式-Ghrelin和去正辛酰Ghrelin，而Ghrelin激活生长激素促分泌素(GHS)受体表达的细胞，而未修饰的Des-n-octanyl形式的Ghrelin不激活Ghrelin。除了这些发现，我们的放射免疫分析系统还显示胃和小肠中有高浓度的Ghrelin，(C)2000学术出版社。
Ghrelin, a novel peptide purified from stomach, is the endogenous ligand for the growth hormone secretagogue receptor and has potent growth hormone-releasing activity. The Ser3 residue of ghrelin is modified by n-octanoic acid, a modification necessary for hormonal activity. We established two ghrelin-specific radioimmunoassays; one recognizes the octanoyl-modified portion and another the C-terminal portion of ghrelin, Using these radioimmunoassay systems, we found that two major molecular forms exist-ghrelin and des-n-octanoyl ghrelin, While ghrelin activates growth-hormone secretagogue (GHS) receptor-expressing cells, the nonmodified des-n-octanyl form of ghrelin, designated as des-acyl ghrelin, does not. In addition to these findings, our radioimmunoassay systems also revealed high concentrations of ghrelin in the stomach and small intestine, (C) 2000 Academic Press.