CHARACTERIZATION OF 2 BARLEY GENES THAT RESPOND RAPIDLY TO DEHYDRATION STRESS
CHARACTERIZATION OF 2 BARLEY GENES THAT RESPOND RAPIDLY TO DEHYDRATION STRESS
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DOI：
10.1016/0168-9452(94)04043-g
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发表时间：
1995-01-01
期刊：
影响因子：
5.2
通讯作者：
CATTIVELLI, L
中科院分区：
文献类型：
--
作者：
GROSSI, M;GULLI, M;CATTIVELLI, L
本研究对大麦基因克隆paf93和cdr29进行了研究，这两个克隆对应于受低温和干旱胁迫调控但不受外源ABA处理调控的mRNAs。在脱水的早期阶段，这些mRNAs在ABA调节的基因如脱水蛋白诱导之前表达，当ABA含量仅有少量增加时，cdr29与两类在大小和表达模式上不同的mRNAs杂交，序列分析表明，paf93编码的蛋白与拟南芥的冷调节蛋白COR47同源，而cdr29代表的是与酵母和哺乳动物编码酰辅酶A氧化酶的同源序列的植物基因。
The present study investigates the barley cDNA clones paf93 and cdr29 which correspond to mRNAs regulated by low temperature and drought stress but not by exogenous ABA treatment. During the early stage of dehydration, these mRNAs are expressed before the induction of known ABA regulated genes such as dehydrins and when only a small increase occurs in ABA content, The cDNA clone cdr29 cross-hybridizes with two classes of mRNAs differing both in size and expression patterns, The sequence analysis revealed that paf93 encodes a protein homologous with the cold-regulated protein COR47 of Arabidopsis whereas cdr29 represents a plant gene homologous to yeast and mammalian sequences coding for acyl-coenzyme A oxidase.