DNA sequence analysis of human chromosome 21 notI linking clones.
DNA sequence analysis of human chromosome 21 notI linking clones.
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人类 21 号染色体 notI 连接克隆的 DNA 序列分析。
DOI：
10.1006/geno.1993.1455
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发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Smith,CL
中科院分区：
文献类型：
--
作者：
Zhu,Y;Cantor,CR;Smith,CL
对16个21号染色体NotI连接克隆的部分序列进行了测定。这些连接的克隆序列代表了序列标记的限制性位点，这些位点对于发现基因和更精细的基因组作图和测序可能是有用的。所有克隆都富含G+C（54 - 83%）。CpG和GpC二核苷酸频率非常接近基于碱基组成的预期值，并且在15个克隆中非常相似。大多数NotI连接克隆来自CpG岛，这通常与基因相关。5个NotI连接克隆具有编码区的高潜力;另外7个克隆也可能含有编码区。NotI连接克隆有许多短的同源区域，但彼此之间或与GenBank序列之间没有广泛的同源性。
Portions of 16 chromosome 21NotI linking clones were sequenced. These linking clone sequences represent sequence-tagged restriction sites that are potentially useful for finding genes and for finer genome mapping and sequencing. All of the clones were G+C rich (54 to 83%). CpG and GpC dinucleotide frequencies were very close to the expected values based on base composition and were very similar in 15 of the clones. Most of theNotI linking clones were derived from CpG islands, which are often associated with genes. FiveNotI linking clones had a high potential for coding regions; 7 additional clones may also contain coding regions. TheNotI linking clones had many short homologous regions, but no extensive homologies either with each other or with GenBank sequences.