Growth and morphological transformations of Helicobacter pylori in broth media
Growth and morphological transformations of Helicobacter pylori in broth media
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DOI：
10.1128/jcm.35.11.2918-2922.1997
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发表时间：
1997-11-01
影响因子：
9.4
通讯作者：
Puszkin, EG
中科院分区：
文献类型：
--
作者：
Andersen, AP;Elliott, DA;Puszkin, EG
幽门螺杆菌是消化性溃疡疾病和某些类型胃癌的病因，通常在不同的琼脂基上培养，需要在37℃下生长2-4天。我们开发了一种新型的肉汤培养基，由2%新生小牛血清、镁、铜、铁、锌、锰和每毫升1毫克裂解的人红细胞组成的基础培养基组分，支持幽门螺杆菌的快速生长，倍增时间约为50分钟。在高于其他革兰氏阴性菌支持菌的pH范围内(pH 8.5)，幽门螺杆菌生长最好，在此添加的肉汤中培养的幽门螺杆菌保持了组织切片和琼脂培养物中的螺旋形态，并保持了较高的尿素酶活性，在该肉汤中培养18小时后，幽门螺杆菌转变为完全失去尿素酶活性的球菌，以前曾报道这些球菌衰老，因为它们不能在琼脂培养基上传代，我们的实验表明，当在新鲜的微氧肉汤中传代时，一些球菌可以恢复到螺旋形态，完全恢复尿素酶活性。
Helicobacter pylori, a cause of peptic ulcer disease and certain types of gastric cancers, has usually been cultured on diverse agar-based media, resulting in a requirement for 2 to 4 days of growth at 37 degrees C, We have developed a novel broth medium consisting of a base medium supplemented with 2% newborn calf serum, Mg2+, Cu2+, Fe2+, Zn2+, Mn2+, and 1 mg of lysed human erythrocytes per ml, This medium supports rapid growth of H. pylori, with a doubling time of about 50 min. Optimal growth was obtained in a pH range higher than that supporting mast other gram-negative bacteria (at pH 8.5), H. pylori cultured in this supplemented broth retains the spiral morphology seen in both histological sections and cultures from agar-based media and also retains a high urease activity, After 18 h in this broth, H. pylori transforms to a coccal form with a complete loss of urease activity, Previously these cocci have been reported to be senescent, since they could not be subcultured on agar medium, Our experiments suggest that some of the cocci can revert back to the spiral morphology with full recovery of urease activity when subcultured in fresh microaerobic broth medium.