Paenibacillus hunanensis sp. nov., isolated from rice seeds.
Paenibacillus hunanensis sp. nov., isolated from rice seeds.
复制标题
DOI：
10.1099/ijs.0.012179-0
复制
发表时间：
2010-06
影响因子：
2.8
通讯作者：
Yang Liu;Lin Liu;Fubin Qiu;P. Schumann;Yu-Ching Shi;Yuanyuan Zou;Xiaoxia Zhang;Wei-bo Song
中科院分区：
文献类型：
--
作者：
Yang Liu;Lin Liu;Fubin Qiu;P. Schumann;Yu-Ching Shi;Yuanyuan Zou;Xiaoxia Zhang;Wei-bo Song
从杂交水稻（Oryza sativa L.）种子中分离到4株革兰氏染色阳性、需氧或兼性厌氧、运动、内孢子形成、杆状细菌，命名为FeL 05（T）、FeL 11、Fek 19和Fek 21。金优611），并采用多相分类法确定了它们的分类地位。基于16 S rRNA基因序列的系统发育分析表明，这4株菌属于类芽孢杆菌属（Paenibacillus）。它们与其他类芽孢杆菌属物种的菌株显示95.4%或更低的序列相似性。发现菌株FeL 05（T）的G+C含量为53.3mol%。其主要的呼吸醌是MK-7。主要的细胞脂肪酸是反异C（15：0）（61.7%）、C（16：0）（10.9%）、异C（16：0）（7.0%）、反异C（17：0）（6.7%）和异C（15：0）（5.2%）。根据其表型特性和系统发育的独特性，菌株FeL 05（T）代表类芽孢杆菌属的一个新物种，并建议将其命名为湖南类芽孢杆菌（Paenibacillus hunanensis sp. nov.）。模式菌株为菌株FeL 05（T）（=ACCC 10718（T）=CGMCC 1.8907（T）=DSM 22170（T））。
Four Gram-stain-positive, aerobic or facultatively anaerobic, motile, endospore-forming, rod-shaped bacteria, designated strains FeL05(T), FeL11, Fek19 and Fek21, were isolated from seeds of hybrid rice (Oryza sativa L. Jinyou 611), and their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four strains were members of the genus Paenibacillus. They showed 95.4 % sequence similarity or less with strains of other Paenibacillus species. The G+C content of strain FeL05(T) was found to be 53.3 mol%. Its predominant respiratory quinone was MK-7. The predominant cellular fatty acids were anteiso-C(15 : 0) (61.7 %), C(16 : 0) (10.9 %), iso-C(16 : 0) (7.0 %), anteiso-C(17 : 0) (6.7 %) and iso-C(15 : 0) (5.2 %). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain FeL05(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus hunanensis sp. nov. is proposed. The type strain is strain FeL05(T) (=ACCC 10718(T) =CGMCC 1.8907(T) =DSM 22170(T)).