Highly amphiphilic manganese porphyrin for the mitochondrial targeting antioxidant.
Highly amphiphilic manganese porphyrin for the mitochondrial targeting antioxidant.
复制标题
高度两亲性的锰卟啉作为线粒体靶向抗氧化剂。
DOI：
10.1093/jb/mvp172
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发表时间：
2010
影响因子：
2.7
通讯作者：
H. Kawakami
中科院分区：
文献类型：
--
作者：
Takayuki Haruyama;S. Asayama;H. Kawakami
在此，我们交流的抗氧化剂的设计组成的两亲性锰卟啉与一个二甲基咪唑基团和三个苯基基团，为靶向的抗氧化剂。结果表明，Mn MImP（3）P具有很高的分配系数（logP（ow）= +4.78），以及显著的超氧化物歧化酶和过氧亚硝酸根分解活性。因此，MnMImP（3）P表现出归因于3，3 '-二丙基-2，2'-硫代二氮杂菁碘化物[diS-C（3）-（5）]的荧光强度的略微增加，所述碘化3，3 '-二丙基-2，2'-硫代二氮杂菁碘化物[diS-C（3）-（5）]是用于评估线粒体膜电位的示踪染料，这表明MnMImP（3）P与线粒体的相互作用导致荧光染料从线粒体膜释放。结果，MnMImP（3）P挽救了与线粒体损伤有关的氧化应激下的细胞死亡。
Here, we communicate that the design of the anti-oxidant consisting of amphiphilic manganese porphyrin with one dimethylimidazolium group and three phenyl groups for mitochondrion targeting. The resulting Mn-porphyrin MnMImP(3)P exhibited high partition coefficient (logP(ow) = +4.78) as well as significant superoxide dismutase and peroxynitrite decomposition activities. Accordingly, the MnMImP(3)P exhibited a little increase in fluorescence intensity attributed to 3,3'-dipropyl-2,2'-thiadicarbocyanine iodide [diS-C(3)-(5)], a tracer dye to assess the mitochondrial membrane potential, which suggested the interaction of the MnMImP(3)P, leading to the release of the fluorescence dye from the mitochondrial membrane, with the mitochondria. As a result, the MnMImP(3)P rescued the cell death under oxidative stress concerned with mitochondrial damage.