ANTIBACTERIAL PEPTIDES FROM PIG INTESTINE - ISOLATION OF A MAMMALIAN CECROPIN
ANTIBACTERIAL PEPTIDES FROM PIG INTESTINE - ISOLATION OF A MAMMALIAN CECROPIN
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DOI：
10.1073/pnas.86.23.9159
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发表时间：
1989-12-01
影响因子：
11.1
通讯作者：
BOMAN, HG
中科院分区：
文献类型：
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作者：
LEE, JY;BOMAN, A;BOMAN, HG
猪小肠用作分批分离富含对大肠杆菌（抗Ec因子）和巨大芽孢杆菌（抗Bm因子）的抗菌活性的肽级分的起始材料。通过不同类型的色谱进行分离和进一步纯化。序列分析表明抗Bm因子与血管活性肠肽相似。发现抗Ec因子具有类似天蚕素的31个残基序列。通过固相合成法确定了其结构，命名为天蚕素P1。在8种不同的细菌上测定了具有和不具有C-末端酰胺的合成天蚕素P1。合成和天然天蚕素P1之间的流动性比较表明，天然肽具有游离的C-末端羧基。
Pig small intestine was used as starting material for a batchwise isolation of a peptide fraction enriched in antibacterial activities against Escherichia coli (anti-Ec factor) and against Bacillus megaterium (anti-Bm factor). Separation and further purification were by different types of chromatography. Sequence analysis showed the anti-Bm factor to be apparently similar to vasoactive intestinal peptide. The anti-Ec factor was found to have a 31-residue sequence that was cecropin-like. It was named cecropin P1 and its structure was confirmed by solid-phase synthesis. Synthetic cecropin P1 with and without C-terminal amide was assayed on eight different bacteria. Mobility comparison between synthetic and natural cecropin P1 indicates that the natural peptide has a free C-terminal carboxyl group.