Connexin 46 and connexin 50 in selenite cataract
Connexin 46 and connexin 50 in selenite cataract
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DOI：
10.1159/000088527
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发表时间：
2006-01-01
影响因子：
2.1
通讯作者：
Fleschner, CR
中科院分区：
文献类型：
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作者：
Fleschner, CR
本研究的目的是确定透镜间隙连接蛋白Cx46和Cx 50是否随硒诱导的白内障的发生而改变。在年轻的Sprague-Dawley大鼠中，通过单次皮下注射亚硒酸钠诱导白内障;年龄匹配的未注射的大鼠作为对照。用差速和不连续蔗糖梯度离心法从正常和白内障晶状体的皮质和核匀浆中分离膜组分。通过用针对Cx46和Cx 50的抗体探测的蛋白质印迹的定量光密度法分析来自膜组分的尿素不溶性蛋白质的等分试样。在白内障晶状体皮质的主要膜部分中发现了更缓慢迁移的Cx46反应带（代表磷酸化Cx46）的显著减少。有没有显着差异，无论是Cx46或Cx 50与亚硒酸盐白内障在任何膜组分检查的量。这些结果表明，间隙连接功能的改变（Cx46的磷酸化的变化所证明的）可能与亚硒酸盐白内障的发展，但无论是Cx46还是Cx 50是受到良好的特征蛋白水解与亚硒酸盐白内障模型。版权所有（C）2006 S. Karger AG，巴塞尔。
The purpose of this work was to determine if the lens gap junction proteins connexin 46 (Cx46) and connexin 50 (Cx50) were altered with the development of selenite-induced cataract. Cataracts were induced in young Sprague-Dawley rats with a single subcutaneous injection of sodium selenite; age-matched uninjected rats served as controls. Membrane fractions were isolated from homogenates of cortex and nucleus of normal and cataractous lenses by differential and discontinuous sucrose gradient centrifugation. Aliquots of urea-insoluble protein from membrane fractions were analyzed by quantitative densitometry of Western blots probed with antibodies to Cx46 and Cx50. A significant decrease in the more slowly migrating Cx46-reactive band, which represents phosphorylated Cx46, was found in the major membrane fraction of the cortex of cataractous lenses. There was no significant difference in the amounts of either Cx46 or Cx50 associated with selenite cataract in any of the membrane fractions examined. These results suggest that alteration of gap junction function ( as evidenced by the change in phosphorylation of Cx46) may be associated with the development of the selenite cataract, but that neither Cx46 nor Cx50 is subject to the well-characterized proteolysis associated with the selenite cataract model. Copyright (C) 2006 S. Karger AG, Basel.