Chitin particles induce size-dependent but carbohydrate-independent innate eosinophilia
Chitin particles induce size-dependent but carbohydrate-independent innate eosinophilia
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DOI：
10.1189/jlb.1110624
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发表时间：
2011-07-01
影响因子：
5.5
通讯作者：
Shibata, Yoshimi
中科院分区：
文献类型：
--
作者：
Kogiso, Mari;Nishiyama, Akihito;Shibata, Yoshimi
小鼠M Phi，吞噬的CMP发展成M1；这一反应取决于颗粒的大小和化学成分。相比之下，最近的研究得出结论，甲壳素颗粒诱导M2和嗜酸性粒细胞迁移，促进获得性Th2免疫反应，对抗含有甲壳素的微生物或过敏原。这项研究调查了甲壳素颗粒的大小和化学成分的变化是否会导致对甲壳素的这些明显不一致的反应。我们比较了M Phi与CMP、LCB和Sephadex G-100珠子(>；40微米)的反应。珠子被给予了IP。对于WT小鼠和CRTH2缺陷的小鼠，CRTH2是嗜酸性粒细胞趋化物质PGD(2)的受体。与cMP诱导的M1激活不同，I.P.在24小时内给予LCB或Sephadex微球可诱导依赖CRTH2的腹膜嗜酸粒细胞增多，以及表达M2表型Arg I的非CRTH2依赖的腹膜M Phi的激活。LCB诱导的M Phi表现为Arg I和表面MR升高，表面TLR2水平降低，CHI3L1和IL-10的产生水平没有变化。我们的结果表明，甲壳素在体内的作用高度依赖于颗粒大小，大的、不能吞噬的珠子，与它们的化学成分无关，诱导固有的嗜酸性粒细胞增多，并激活表达几种M2表型而不是M1表型的M-Phi。J.Leukoc。比奥尔。90：167-176；2011。
Murine M phi that phagocytose CMP develop into M1; this response depends on the size and the chemical composition of the particles. In contrast, recent studies concluded that chitin particles induce M2 and eosinophil migration, promoting acquired Th2 immune responses against chitin-containing microbes or allergens. This study examined whether these apparently inconsistent responses to chitin could be induced by variation in the size and chemical composition of the chitin particles. We compared the responses of M phi with CMP, LCB, and Sephadex G-100 beads (>40 mu m). Beads were given i.p. to WT mice and to mice deficient in a CRTH2, a receptor for the eosinophil chemoattractant PGD(2). In contrast to the M1 activation induced by CMP, i.p. administration of LCB or Sephadex beads induced within 24 h a CRTH2-dependent peritoneal eosinophilia, as well as CRTH2-independent activation of peritoneal M phi that expressed Arg I, an M2 phenotype. LCB-induced M phi exhibited elevated Arg I and a surface MR, reduced surface TLR2 levels, and no change in the levels of CHI3L1 or IL-10 production. Our results indicate that the effects of chitin in vivo are highly dependent on particle size and that large, nonphagocytosable beads, independent of their chemical composition, induce innate eosinophilia and activate M phi expressing several M2, but not M1, phenotypes. J. Leukoc. Biol. 90: 167-176; 2011.