Control of root cap formation by microRNA-targeted auxin response factors in Arabidopsis
Control of root cap formation by microRNA-targeted auxin response factors in Arabidopsis
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DOI：
10.1105/tpc.105.033076
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发表时间：
2005-08-01
期刊：
影响因子：
11.6
通讯作者：
Chen, XY
中科院分区：
文献类型：
--
作者：
Wang, JW;Wang, LJ;Chen, XY
植物根冠调节根尖的生长方向并保护内部细胞。根冠细胞由远端干细胞连续产生，植物激素生长素为根远端组织提供位置信息。在这里，我们确定拟南芥生长素反应因子ARF 10和ARF 16，microRNA 160（miR 160）为目标，作为根冠细胞形成的控制器。Pro（35S）：在MIR 160植物中，其中ARF 10和ARF 16的表达被抑制，并且arf 10 - 2和arf 16 - 2双突变体显示出相同的根尖缺陷，在根远端区域中具有不受控制的细胞分裂和受阻的细胞分化，并且显示出肿瘤样根尖和重力感丧失。ARF 10和ARF 16在限制干细胞生态位和促进柱细胞分化方面发挥作用;虽然功能冗余，但这两个ARF对于根冠发育是不可或缺的，并且生长素信号不能绕过它们来启动柱细胞产生。在根中，生长素和miR 160独立地调节ARF 10和ARF 16基因的表达，产生与根冠发育一致的模式。我们进一步证明了miR 160非偶联的ARF 16产生对植物表型的多效性作用，miR 160在调节拟南芥的发育和生长中起着重要作用。
The plant root cap mediates the direction of root tip growth and protects internal cells. Root cap cells are continuously produced from distal stem cells, and the phytohormone auxin provides position information for root distal organization. Here, we identify the Arabidopsis thaliana auxin response factors ARF10 and ARF16, targeted by microRNA160 ( miR160), as the controller of root cap cell formation. The Pro(35S): MIR160 plants, in which the expression of ARF10 and ARF16 is repressed, and the arf10- 2 arf16- 2 double mutants display the same root tip defect, with uncontrolled cell division and blocked cell differentiation in the root distal region and show a tumor- like root apex and loss of gravity- sensing. ARF10 and ARF16 play a role in restricting stem cell niche and promoting columella cell differentiation; although functionally redundant, the two ARFs are indispensable for root cap development, and the auxin signal cannot bypass them to initiate columella cell production. In root, auxin and miR160 regulate the expression of ARF10 and ARF16 genes independently, generating a pattern consistent with root cap development. We further demonstrate that miR160- uncoupled production of ARF16 exerts pleiotropic effects on plant phenotypes, and miR160 plays an essential role in regulating Arabidopsis development and growth.