Processing enzyme ribonuclease E specifically cleaves RNA I. An inhibitor of primer formation in plasmid DNA synthesis.
Processing enzyme ribonuclease E specifically cleaves RNA I. An inhibitor of primer formation in plasmid DNA synthesis.
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DOI：
10.1016/0022-2836(85)90056-7
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发表时间：
1985-10
影响因子：
5.6
通讯作者：
T. Tomcsanyi;D. Apirion
中科院分区：
文献类型：
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作者：
T. Tomcsanyi;D. Apirion
当携带大肠杆菌素E1质粒衍生物的大肠杆菌中RNA加工酶RNAase E失活时，一个约100个核苷酸长的小RNA会积累起来。对该RNA的结构分析表明，它是抑制质粒DNA合成的RNA I。RNA I是RNAase E的特异性底物，切割发生在分子5 '端第5和第6个核苷酸之间。这是迄今为止发现的第二种天然RNA底物，用于RNA加工酶核糖核酸酶E，另一种是5s核糖体RNA的前体。非凡的,9个核苷酸在乳沟网站是相同的在这两种基质:CG↓UUUGp。因此，我们认为RNAase E与其底物之间的相互作用至少部分是由这9个核苷酸控制的。
When the RNA processing enzyme RNAase E is inactivated in anEscherichia colistrain carrying derivatives of the colicin E1 plasmid, a small RNA, about 100 nucleotides long, accumulates. Structural analysis of this RNA showed that it is RNA I, the RNA that inhibits plasmid DNA synthesis. RNA I is a specific substrate for RNAase E and the cleavage takes place between the fifth and sixth nucleotides from the 5′ end of the molecule. This is only the second natural RNA substrate that has been found, so far, for the RNA processing enzyme ribonuclease E, the other being a precursor for 5 S ribosomal RNA. It is remarkable that nine nucleotides around the cleavage sites are identical in both substrates: ACAGA↓UAUUUGp. Therefore, we suggest that at least part of the interaction between RNAase E and its substrate is controlled by these nine nucleotides.