Serum Epstein-Barr virus DNA load in primary Epstein-Barr virus infection
Serum Epstein-Barr virus DNA load in primary Epstein-Barr virus infection
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DOI：
10.1002/jmv.20237
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发表时间：
2005-01-01
影响因子：
12.7
通讯作者：
Puchhammer-Stöckl, E
中科院分区：
文献类型：
--
作者：
Bauer, CC;Aberle, SW;Puchhammer-Stöckl, E
原发性EB病毒(EBV)感染的特异性病毒实验室诊断通常基于抗体检测分析。在感染的急性裂解期，也可以在血清中检测到病毒DNA。本研究旨在探讨血清EB病毒DNA检测和定量检测对原发性EBV感染的诊断价值。采用实时荧光定量聚合酶链式反应技术检测了98例年龄147岁、有症状、抗体阳性的EB病毒原发感染患者的血清EBVDNA水平。研究了病毒载量与发病时间、年龄以及临床和实验室数据之间的关系。定量聚合酶链式反应检测EBV DNA 93例(94.9%)，病毒载量在3.8×10(1)~6.6×10(4)拷贝/ml之间，EBV DNA检测对原发性EBV感染的敏感性为94.9%，特异性为97.4%。直到出现症状的第12天，EBV DNA才能被检测到，而在发病后的22天内，没有发现进一步的阳性的PCR结果。EB病毒DNA的检测与疾病的临床表现的相关性也比低亲和力的EBV特异性VCA抗体的存在更明显。EB病毒DNA载量与发病时间呈负相关(P
Specific viral laboratory diagnosis of primary Epstein-Barr Virus (EBV) infection is usually based on antibody-detection assays. During acute, lytic phase of infection, viral DNA can also be detected in serum. In the present study, the diagnostic utility of EBV DNA detection and quantitation in serum in primary EBV infection was investigated. The level of EBV DNA in the serum of 98 immunocompetent patients aged 147 years with symptomatic, anti body-confirmed EBV primary infection was assessed using a quantitative real-time PCR assay. The association between viral load and time after onset of disease, age and clinical and laboratory data was investigated. Quantitative PCR detected EBV DNA in 93 of 98 samples (94.9%), and the measured viral loads ranged from 3.8 x 10(1) to 6.6 x 10(4) copies/ml. EBV DNA detection exhibited a sensitivity of 94.9% and a specificity of 97.4% for primary EBV infection. EBV DNA was always detectable until day 12 after onset of symptoms, whereas no further positive PCR results were found after a period of 22 days after onset of disease. Detection of EBV DNA also showed a clearer association with the clinical manifestation of disease than the presence of EBV specific VCA IgG antibodies of low avidity. EBV DNA load was found to correlate inversely with the time after onset of disease (P