Novel Bio-functional Magnesium Coating on Porous Ti6Al4V Orthopaedic Implants: In vitro and In vivo Study.
Novel Bio-functional Magnesium Coating on Porous Ti6Al4V Orthopaedic Implants: In vitro and In vivo Study.
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多孔 Ti6Al4V 骨科植入物上的新型生物功能镁涂层：体外和体内研究
DOI：
10.1038/srep40755
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发表时间：
2017-01-19
影响因子：
4.6
通讯作者：
Guo Z
中科院分区：
文献类型：
--
作者：
Li X;Gao P;Wan P;Pei Y;Shi L;Fan B;Shen C;Xiao X;Yang K;Guo Z
具有各种多孔结构的钛及其合金是骨科植入物中使用的最重要的金属之一，因为其具有良好的硬组织替代性能。然而，表面改性是提高钛及其合金骨结合的关键。采用电弧离子镀技术在多孔Ti6 Al 4V表面制备了具有生物活性的镁涂层，该涂层具有晶粒细小、膜基结合力强等特点。用X射线衍射仪和扫描电子显微镜（SEM）结合能谱仪对其表面组成和形貌进行了表征。体外细胞毒性和MC 3 T3-E1细胞增殖实验表明，镁涂层多孔Ti6 Al 4V具有良好的降解性和生物相容性。此外，荧光标记、显微计算机断层扫描分析扫描和组织切片Van-Gieson染色等体内研究表明，镁涂层多孔Ti6 Al 4V在植入4周和8周后可明显促进兔股骨髁缺损的骨再生，并且比裸多孔Ti6 Al 4V具有更好的成骨和骨整合能力。因此，可以预期这种具有改善的骨整合和成骨功能的多孔Ti6 Al 4V支架上的生物活性镁涂层可用于骨科应用。
Titanium and its alloys with various porous structures are one of the most important metals used in orthopaedic implants due to favourable properties as replacement for hard tissues. However, surface modification is critical to improve the osteointegration of titanium and its alloys. In this study, a bioactive magnesium coating was successfully fabricated on porous Ti6Al4V by means of arc ion plating, which was proved with fine grain size and high film/substrate adhesion. The surface composition and morphology were characterized by X-ray diffraction and SEM equipped with energy dispersive spectroscopy. Furthermore, the in vitro study of cytotoxicity and proliferation of MC3T3-E1 cells showed that magnesium coated porous Ti6Al4V had suitable degradation and biocompatibility. Moreover, the in vivo studies including fluorescent labelling, micro-computed tomography analysis scan and Van-Gieson staining of histological sections indicated that magnesium coated porous Ti6Al4V could significantly promote bone regeneration in rabbit femoral condylar defects after implantation for 4 and 8 weeks, and has better osteogenesis and osteointegration than the bare porous Ti6Al4V. Therefore, it is expected that this bioactive magnesium coating on porous Ti6Al4V scaffolds with improved osteointegration and osteogenesis functions can be used for orthopedic applications.