Characterization of a new highly mosquitocidal isolate of Bacillusthuringiensis--an alternative to Bti?
Characterization of a new highly mosquitocidal isolate of Bacillusthuringiensis--an alternative to Bti?
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DOI：
10.1016/j.jip.2011.11.003
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发表时间：
2012-02
影响因子：
3.4
通讯作者：
Wenfei Zhang;N. Crickmore;Z. George;L. Xie;Yong-Qiang He;Youzhi Li;Ji-Liang Tang;L. Tian;Xi Wang;X. Fang
中科院分区：
文献类型：
--
作者：
Wenfei Zhang;N. Crickmore;Z. George;L. Xie;Yong-Qiang He;Youzhi Li;Ji-Liang Tang;L. Tian;Xi Wang;X. Fang
蚊子是一种非常重要的媒介，参与致病病毒和寄生虫的全球传播。控制蚊子种群仍然是预防疟疾、黄热病、登革热、丝虫病等严重传染病的最佳手段之一，并且开发生物杀虫剂作为化学杀虫剂的有用替代品的兴趣越来越大。结果表明，苏云金芽孢杆菌苏云金亚种（Bacillus thuringiensis subsp.）Israelensis（Bti）由于其对多种蚊幼虫的特异性和高毒性而被广泛使用。然而，谨慎的做法是寻找具有替代杀蚊活性谱的Bti替代品或能够克服可能对Bti产生的任何抗性的替代品。Bt S2160-1菌株是从中国南方采集的土壤样品中分离得到的，具有与Bti相当的杀蚊活性。但它们的质粒谱、产生的晶体蛋白和cry基因互补性存在显着差异。利用PCR-限制性片段长度多态性技术，在Bt S2160-1中鉴定出4个新的cry基因（cry 30 Ea、cry 30 Ga、cry 50 Ba和cry 54 Ba）。因此，Bt S2160-1可作为Bti的潜在替代品，用于控制蚊虫种群，降低蚊媒疾病的发病率。
The mosquito is a very important vector involved in the worldwide transmission of disease-causing viruses and parasites. Controlling the mosquito population remains one of the best means for preventing the serious infectious diseases of malaria, yellow fever, dengue, filariasis and so on and there has been an increasing interest in developing biopesticides as a useful substitute to chemical insecticides. As a result, Bacillus thuringiensis subsp. israelensis (Bti) has been extensively used due to its specificity and high toxicity to a variety of mosquito larvae. However it is prudent to seek alternatives to Bti with alternative spectra of mosquitocidal activity or that are able to overcome any resistance that might develop against Bti. The Bt S2160-1 strain was isolated from soil samples collected from Southern China and found to have a comparable mosquitocidal activity to Bti. However there were significant differences in terms of their plasmid profiles, crystal proteins produced and cry gene complement. A PCR-restriction fragment length polymorphism identification system was developed and used in order to identify novel cry-type genes and four such genes (cry30Ea, cry30Ga, cry50Ba and cry54Ba) were identified in Bt S2160-1. In conclusion, Bt S2160-1 has been identified as a potential alternative to Bti, which could be used for the control of mosquito populations in order to reduce the incidence of mosquito-borne diseases.