Effects of avian infectious bronchitis virus antigen on eggshell formation and immunoreaction in hen oviduct
Effects of avian infectious bronchitis virus antigen on eggshell formation and immunoreaction in hen oviduct
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DOI：
10.1016/j.theriogenology.2014.02.002
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发表时间：
2014-05-01
期刊：
影响因子：
2.8
通讯作者：
Yoshimura, Yukinori
中科院分区：
文献类型：
--
作者：
Nii, Takahiro;Isobe, Naoki;Yoshimura, Yukinori
本研究的目的是确定鸡传染性支气管炎病毒（IBV）影响蛋壳形成的机制。将减毒的IBV（aIBV组）或媒介物（对照组）注射到白色来航产蛋鸡的输卵管大管腔中。蛋壳形成相关基因表达的变化细胞毒性细胞（CD 8（+）和TCR-γ δ（+）T细胞）的密度，以及与细胞毒性免疫反应相关的分子的基因表达（B-NK、穿孔素、颗粒酶和IL-2）和促炎细胞因子通过定量逆转录聚合酶链反应或免疫组化检测峡部和子宫中的IL-1 β、IL-6和IFN-γ。通过逆转录聚合酶链反应分析峡部和子宫的管状腺细胞中IL-1 β和IL-6受体的基因表达。与对照组相比，aIBV组子宫峡部I型胶原和子宫CaBP-D28 K的基因表达降低，但不表达V型胶原。aIBV组峡部和子宫中CD 8（+）细胞和TCR-γ δ（+）T细胞的频率显著高于对照组。细胞毒性分子和促炎细胞因子的表达也高于对照组。在峡部和子宫的管状腺细胞中鉴定到IL-6受体的表达，而不是IL-1 β受体。这些结果表明，IBV感染引起蛋壳形成的障碍，通过干扰基因表达的I型胶原在峡部和CaBP-D28 K在子宫，可能通过细胞毒性细胞和促炎细胞因子的物质的影响。(C)2014爱思唯尔公司All rights reserved.
The aim of this study was to determine the mechanism by which the avian infectious bronchitis virus (IBV) affects eggshell formation. Attenuated IBV (aIBV group) or vehicle (control group) was injected into the oviductal magnum lumen of White Leghorn laying hens. The changes in the expression of genes related to eggshell formation (collagen types land V, and CaBP-D28K), densities of cytotoxic cells (CD8(+) and TCR-gamma delta(+) Tcells), and gene expression of molecules related to cytotoxic immunoreaction (B-NK, perforin, granzyme, and IL-2) and proinflammatory cytokines (IL-1 beta, IL-6 and IFN-gamma) were examined by quantitative reverse transcriptase polymerase chain reaction or immunohistochemistry in the isthmus and uterus. Gene expression of IL-1 beta and IL-6 receptors in the tubular gland cells of the isthmus and uterus was analyzed by reverse transcriptase polymerase chain reaction. Gene expression of collagen type I, but not collagen type V, in the isthmus and CaBP-D28K in the uterus was decreased in the aIBV group compared with that in the control. The frequencies of CD8(+) cells and TCR-gamma delta(+) T cells in the isthmus and uterus were significantly higher in the aIBV group than in the control group. The expression of cytotoxic molecular and proinflammatory cytokines was also higher in the aIBV group than in the control. The expression of IL-6 receptor, but not IL-1 beta receptor, was identified in the tubular gland cells in the isthmus and uterus. These results suggest that IBV infection causes disorder of eggshell formation by disturbing gene expression of collagen type I in the isthmus and CaBP-D28K in the uterus, probably via the effects of substances from cytotoxic cells and proinflammatory cytokines. (C) 2014 Elsevier Inc. All rights reserved.