The total number of white matter interstitial neurons in the human brain
The total number of white matter interstitial neurons in the human brain
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DOI：
10.1111/joa.13018
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发表时间：
2019-09-01
影响因子：
2.4
通讯作者：
Judas, Milos
中科院分区：
文献类型：
--
作者：
Sedmak, Goran;Judas, Milos
在成人大脑中，皮质下白色物质的间质神经元（WMIN）是胎儿板下区的残存物。有人认为，他们执行某些重要的功能，并可能参与一些神经和精神疾病的发病机制。然而，这类人类皮层神经元的许多重要特征仍然没有得到充分的探索。在这项研究中，我们分析了总数，区域和拓扑分布的WMIN在成人皮质下白色的问题，使用相结合的免疫细胞化学（NeuN）和体视学方法。我们发现，1 mm（3）皮质下白色物质中WMIN的平均数量为1.230 +/- 549，这意味着整个皮质下端脑白色物质中WMIN的平均总数为593 811 183.6 +/- 264 849 443.35。虽然它们的区域分布没有显著差异，但在边缘皮层中观察到的WMIN数量最少，而在额叶皮层中观察到的WMIN数量最多。就其拓扑分布而言，WMIN在脑回冠部内的数量始终较多，沿脑回壁沿着的数量较少，在皮质沟底部的数量最少（在皮质-白质边界下方的狭窄致密区）。WMIN的拓扑位置也与它们的形态显著相关：锥体和多极形式是脑回冠内最多的，而双极形式在皮质沟底部占主导地位。我们的研究结果表明，WMIN代表大量的神经元群体在成人大脑皮层（例如，更多的丘脑或基底节神经元），因此值得更详细的形态和功能的调查在未来。
In the adult human brain, the interstitial neurons (WMIN) of the subcortical white matter are the surviving remnants of the fetal subplate zone. It has been suggested that they perform certain important functions and may be involved in the pathogenesis of several neurological and psychiatric disorders. However, many important features of this class of human cortical neurons remain insufficiently explored. In this study, we analyzed the total number, and regional and topological distribution of WMIN in the adult human subcortical white matter, using a combined immunocytochemical (NeuN) and stereological approaches. We found that the average number of WMIN in 1 mm(3) of the subcortical white matter is 1.230 +/- 549, which translates to the average total number of 593 811 183.6 +/- 264 849 443.35 of WMIN in the entire subcortical telencephalic white matter. While there were no significant differences in their regional distribution, the lowest number of WMIN has been consistently observed in the limbic cortex, and the highest number in the frontal cortex. With respect to their topological distribution, the WMIN were consistently more numerous within gyral crowns, less numerous along gyral walls and least numerous at the bottom of cortical sulci (where they occupy a narrow and compact zone below the cortical-white matter border). The topological location of WMIN is also significantly correlated with their morphology: pyramidal and multipolar forms are the most numerous within gyral crowns, whereas bipolar forms predominate at the bottom of cortical sulci. Our results indicate that WMIN represent substantial neuronal population in the adult human cerebral cortex (e.g. more numerous than thalamic or basal ganglia neurons) and thus deserve more detailed morphological and functional investigations in the future.