Phosphorylated RNA polymerase II stimulates pre-mRNA splicing
Phosphorylated RNA polymerase II stimulates pre-mRNA splicing
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DOI：
10.1101/gad.13.10.1234
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发表时间：
1999-05-15
影响因子：
10.5
通讯作者：
Manley, JL
中科院分区：
文献类型：
--
作者：
Hirose, Y;Tacke, R;Manley, JL
RNA聚合酶II（RNAP II）负责真核细胞中mRNA前体的转录。然而，最近的研究表明，RNAP II也参与随后的RNA加工反应，通过羧基末端结构域（CTD）的RNAP II最大亚基和加工因子之间的相互作用。使用重建的体外剪接试验，我们表明RNAP II的功能直接在前mRNA剪接的影响非常早期的步骤中组装的剪接体。我们证明，CTD的磷酸化状态显着影响活动：高度磷酸化的RNAP IIO强烈激活剪接，而hypophosphorylated RNAP IIA可以抑制反应。
RNA polymerase II (RNAP II) is responsible for transcription of mRNA precursors in eukaryotic cells. Recent studies, however, have suggested that RNAP II also participates in subsequent RNA processing reactions through interactions between the carboxy-terminal domain (CTD) of the RNAP II largest subunit and processing factors. Using reconstituted in vitro splicing assays, we show that RNAP II functions directly in pre-mRNA splicing by influencing very early steps in assembly of the spliceosome. We demonstrate that the phosphorylation status of the CTD dramatically affects activity: Hyperphosphorylated RNAP IIO strongly activates splicing, whereas hypophosphorylated RNAP IIA can inhibit the reaction.