Skeletal muscle capillary density is related to anaerobic threshold and claudication in peripheral artery disease.
Skeletal muscle capillary density is related to anaerobic threshold and claudication in peripheral artery disease.
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DOI：
10.1177/1358863x20945794
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Annex BH
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文献类型：
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作者：
Duscha BD;Kraus WE;Jones WS;Robbins JL;Piner LW;Huffman KM;Allen JD;Annex BH
外周动脉疾病（PAD）的特征是下肢血流受损，导致跛行和运动不耐受。运动不耐受可能由骨骼肌毛细血管密度降低和肌肉供氧受损引起。本横断面研究验证了毛细血管密度与PAD患者跛行次数和厌氧阈值（AT）相关的假设。共有37名PAD患者和29名对照组在跑步机上进行心肺运动试验，以进行AT和腓肠肌活检。采用免疫荧光染色法测定骨骼肌毛细血管密度。与对照组相比，PAD降低了毛细血管密度（278±87 vs 331±86内皮细胞/mm2， p = 0.05），峰值VO2（15.7±3.9 vs 24.3±5.2 mL/kg/min, p < 0.001）和at时的VO2（11.5±2.6 vs 16.1±2.8 mL/kg/min, p < 0.001）。PAD患者（非对照组）毛细血管密度与at时VO2 （r = 0.343; p = 0.038）、at前时间（r = 0.381; p = 0.020）、at后至试验终止时间（r = 0.610; p < 0.001）相关。毛细血管密度也与跛行时间（r = 0.332; p = 0.038）和跛行至试验终止时间（r = 0.584; p < 0.001）有关。总之，毛细血管密度、AT和跛行症状之间的关系表明，在PAD中，运动限制可能部分依赖于有限的骨骼肌毛细血管密度和氧化代谢。
Peripheral artery disease (PAD) is characterized by impaired blood flow to the lower extremities, causing claudication and exercise intolerance. Exercise intolerance may result from reduced skeletal muscle capillary density and impaired muscle oxygen delivery. This cross-sectional study tested the hypothesis that capillary density is related to claudication times and anaerobic threshold (AT) in patients with PAD. A total of 37 patients with PAD and 29 control subjects performed cardiopulmonary exercise testing on a treadmill for AT and gastrocnemius muscle biopsies. Skeletal muscle capillary density was measured using immunofluorescence staining. PAD had decreased capillary density (278 ± 87 vs 331 ± 86 endothelial cells/mm2, p = 0.05), peak VO2 (15.7 ± 3.9 vs 24.3 ± 5.2 mL/kg/min, p ⩽ 0.001), and VO2 at AT (11.5 ± 2.6 vs 16.1 ± 2.8 mL/kg/min, p ⩽ 0.001) compared to control subjects. In patients with PAD, but not control subjects, capillary density was related to VO2 at AT (r = 0.343; p = 0.038), time to AT (r = 0.381; p = 0.020), and time after AT to test termination (r = 0.610; p ⩽ 0.001). Capillary density was also related to time to claudication (r = 0.332; p = 0.038) and time after claudication to test termination (r = 0.584; p ⩽ 0.001). In conclusion, relationships between capillary density, AT, and claudication symptoms indicate that, in PAD, exercise limitations are likely partially dependent on limited skeletal muscle capillary density and oxidative metabolism.