The effect of hyaluronic acid and phospholipid based lubricants on friction within a human cartilage damage model
The effect of hyaluronic acid and phospholipid based lubricants on friction within a human cartilage damage model
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DOI：
10.1016/j.biomaterials.2006.04.018
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发表时间：
2006-09-01
期刊：
影响因子：
14
通讯作者：
Ingham, Eileen
中科院分区：
文献类型：
--
作者：
Forsey, Richard W.;Fisher, John;Ingham, Eileen
在体外模型中评估高分子量透明质酸（HA）、二棕榈酰磷脂酰胆碱（DPCC）以及HA和DPCC两者的混合物的不同制剂的润滑能力。使用骨关节炎（OA）损伤的人软骨模型在专门设计的摩擦装置内确定启动摩擦的水平。为了检查HA的长期益处，使用荧光标记的HA和共聚焦显微镜研究HA渗透到软骨组织中的程度。发现在该模型中，与林格氏溶液对照相比，HA的所有制剂和大多数DPCC润滑剂降低摩擦（HA 5和10 mg ml（-1），DPPC 200 mg ml（-1）分别降低51.9%、46.7%和46.5%）。发现HA制剂的润滑性不依赖于浓度，但浓度是DPCC润滑性的关键（100 mg ml（-1）仅减少15.9%的摩擦）。通过组合HA和DPCC（HA/DPPC; 5 mg ml（-1）/100 mg ml（-1）和10 mg ml（-1）/200 mg ml（-1）），注意到进一步的改善（分别为69.5%和61.9%），因为摩擦的平均水平比最有效的单独制剂（HA 5 mg ml（-1））进一步降低高达17%。HA从表面渗透到牛软骨中达300 μ m，观察到超过48 It期。观察到HA特异性靶向软骨细胞，因为它主要存在于细胞周围的陷窝内。(c)2006爱思唯尔有限公司保留所有权利。
The lubricating abilities of different formulations of high molecular weight hyaluronic acid (HA), dipalmitoyl phosphatidylcholine (DPCC) and mixtures of both HA and DPCC were assessed in an in vitro model. Levels of start-up friction were determined using an osteoarthritis (OA) damaged human cartilage model set within a specially designed friction rig. To examine the long term benefits of HA, the extent of penetration of HA into cartilage tissue was investigated using fluorescently labelled HA and confocal microscopy. It was found that in this model, all formulations of HA and the majority of DPCC lubricants reduced friction (HA 5 and 10mg ml(-1), DPPC 200 mg ml(-1) reductions of 51.9%, 46.7% and 46.5% respectively), compared to a Ringers solution control. Lubrication was found not to be concentration dependant for HA formulations, but concentration was key for DPCC lubrication (100mg ml(-1) reduced friction by only 15.9%). By combining HA and DPCC (HA/DPPC; 5 mg ml(-1)/100 mg ml(-1) and 10 mg ml(-1)/200 mg ml(-1)), a further improvement was noted (69.5% and 61.9%, respectively) as the mean levels of friction were reduced by up to a further 17% than the most effective individual formulation (HA 5 mg ml(-1)). Penetration of HA into bovine cartilage by up to 300 mu m from the surface was observed over a 48 It period. It was observed that HA specifically targeted the chondrocytes as it was primarily found within the lacunae surrounding the cells. (c) 2006 Elsevier Ltd. All rights reserved.