Detection of SARS-CoV-2 infection by saliva and nasopharyngeal sampling in frontline healthcare workers: An observational cohort study.
Detection of SARS-CoV-2 infection by saliva and nasopharyngeal sampling in frontline healthcare workers: An observational cohort study.
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DOI：
10.1371/journal.pone.0280908
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
Ferreira, Daniela M. M.
中科院分区：
文献类型：
--
作者：
Walker, Naomi F. F.;Byrne, Rachel L. L.;Howard, Ashleigh;Nikolaou, Elissavet;Farrar, Madlen;Glynn, Sharon;Cheliotis, Katerina S. S.;Atienzar, Ana I. Cubas I.;Davies, Kelly;Reine, Jesus;Rashid-Gardner, Zalina;German, Esther L. L.;Solorzano, Carla;Blandamer, Tess;Hitchins, Lisa;Myerscough, Christopher;Gessner, Bradford D. D.;Begier, Elizabeth;Collins, Andrea M. M.;Beadsworth, Mike;Todd, Stacy;Hill, Helen;Houlihan, Catherine F. F.;Nastouli, Eleni;Adams, Emily R. R.;Mitsi, Elena;Ferreira, Daniela M. M.
SARS-CoV-2 大流行给全球医疗保健系统造成了前所未有的压力，其中包括英国国家医疗服务体系 (NHS)。我们对第一波大流行期间在紧急 NHS Trust 工作的一线医护人员 (HCW) 进行了 SARS-CoV-2 感染观察性队列研究，以回答有关 SARS-CoV-2 感染、诊断、传播和控制的新问题。使用每周自行收集的唾液和每周两次的口咽/鼻咽 (OP/NP) 合并样本，除了自我评估的症状特征和隔离行为之外，我们还回顾性比较了通过 RT-qPCR 对唾液和 OP/NP 样本进行的 SARS-CoV-2 检测。我们报告了与同期症状和隔离行为的关联。自 2020 年 3 月 30 日起的 12 周内，40·0%（n = 34/85，95% 置信区间 31·3–51·8%）医护人员通过监测 OP/NP 拭子和/或唾液样本发现了 SARS-CoV-2 感染的证据。 47·1% (n = 40) 的参与者报告了症状，25·9% (n = 22) 的参与者报告了自我隔离。只有 44.1% (n = 15/34) 的 SARS-CoV-2 感染参与者在检测结果呈阳性后 14 天内报告有任何症状，并且只有 29·4% (n = 10/34) 报告了自我隔离期。配对唾液和 OP/NP 拭子之间的总体一致性为 93·4%（n = 211/226 对），但阳性一致性率较低。在至少有一项阳性结果的配对样本中，35·0% (n = 7/20) 仅通过 OP/NP 拭子检测呈阳性，40·0% (n = 8/20) 仅通过唾液检测呈阳性，只有 25·0% (n = 5/20) OP/NP 和唾液结果均为阳性。医务人员是医院中 SARS-CoV-2 传播的潜在来源，症状筛查将识别少数感染者。如果不进行常规无症状 SARS-CoV-2 筛查，感染 SARS-CoV-2 的医护人员很可能会继续上班。除了 OP/NP 拭子检测之外，唾液有助于确定有症状和无症状的 SARS-CoV-2 感染。结合唾液和 OP/NP 拭子采样将改善 SARS-CoV-2 的检测以进行监测，并建议采用高灵敏度策略。
The SARS-CoV-2 pandemic has caused an unprecedented strain on healthcare systems worldwide, including the United Kingdom National Health Service (NHS). We conducted an observational cohort study of SARS-CoV-2 infection in frontline healthcare workers (HCW) working in an acute NHS Trust during the first wave of the pandemic, to answer emerging questions surrounding SARS-CoV-2 infection, diagnosis, transmission and control. Using self-collected weekly saliva and twice weekly combined oropharyngeal/nasopharyngeal (OP/NP) samples, in addition to self-assessed symptom profiles and isolation behaviours, we retrospectively compared SARS-CoV-2 detection by RT-qPCR of saliva and OP/NP samples. We report the association with contemporaneous symptoms and isolation behaviour. Over a 12-week period from 30th March 2020, 40·0% (n = 34/85, 95% confidence interval 31·3–51·8%) HCW had evidence of SARS-CoV-2 infection by surveillance OP/NP swab and/or saliva sample. Symptoms were reported by 47·1% (n = 40) and self-isolation by 25·9% (n = 22) participants. Only 44.1% (n = 15/34) participants with SARS-CoV-2 infection reported any symptoms within 14 days of a positive result and only 29·4% (n = 10/34) reported self-isolation periods. Overall agreement between paired saliva and OP/NP swabs was 93·4% (n = 211/226 pairs) but rates of positive concordance were low. In paired samples with at least one positive result, 35·0% (n = 7/20) were positive exclusively by OP/NP swab, 40·0% (n = 8/20) exclusively by saliva and in only 25·0% (n = 5/20) were the OP/NP and saliva result both positive. HCW are a potential source of SARS-CoV-2 transmission in hospitals and symptom screening will identify the minority of infections. Without routine asymptomatic SARS-CoV-2 screening, it is likely that HCW with SARS-CoV-2 infection would continue to attend work. Saliva, in addition to OP/NP swab testing, facilitated ascertainment of symptomatic and asymptomatic SARS-CoV-2 infections. Combined saliva and OP/NP swab sampling would improve detection of SARS-CoV-2 for surveillance and is recommended for a high sensitivity strategy.
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影响因子：
9.4
作者：
Lee RA;Herigon JC;Benedetti A;Pollock NR;Denkinger CM
通讯作者：
Denkinger CM
影响因子：
13.8
作者：
通讯作者：
--
DOI：
10.1016/j.jmoldx.2020.12.008
发表时间：
2021-03
期刊：
The Journal of molecular diagnostics : JMD
影响因子：
--
作者：
O'Leary TJ
通讯作者：
O'Leary TJ
DOI：
10.1016/s1473-3099(21)00146-8
发表时间：
2021-09
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Tsang NNY;So HC;Ng KY;Cowling BJ;Leung GM;Ip DKM
通讯作者：
Ip DKM
影响因子：
11.8
作者：
Ott IM;Strine MS;Watkins AE;Boot M;Kalinich CC;Harden CA;Vogels CBF;Casanovas-Massana A;Moore AJ;Muenker MC;Nakahata M;Tokuyama M;Nelson A;Fournier J;Bermejo S;Campbell M;Datta R;Dela Cruz CS;Farhadian SF;Ko AI;Iwasaki A;Grubaugh ND;Wilen CB;Wyllie AL;Yale IMPACT Research team3
通讯作者：
Yale IMPACT Research team3