Evidence for differential localization of two binding sites for L-[3H]glutamate in rat fascia dentata.
Evidence for differential localization of two binding sites for L-[3H]glutamate in rat fascia dentata.
复制标题
大鼠齿状筋膜中 L-[3H]谷氨酸两个结合位点差异定位的证据。
DOI：
10.1016/0006-8993(83)90744-8
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Nadler,JV
中科院分区：
文献类型：
--
作者：
Werling,LL;Nadler,JV
通过测定选择性损伤对特异性结合的影响，初步定位了[~3H]谷氨酸的两个结合部位在大鼠齿状筋膜内。用秋水仙碱破坏齿状颗粒细胞和消融同侧内嗅皮层均显著减少放射配基与准敏感部位(GLU A)的结合，但只有内嗅觉损伤显著减少与对准敏感部位(GLU B)不敏感的部位的结合。这些结果表明，GluA结合部位主要定位在齿状颗粒细胞上，而GluB结合部位部分定位于穿通径纤维上，而不定位于颗粒细胞上。
Two binding sites forl-[3H]glutamate were tentatively localized in rat fascia dentata by determining the effects of selective lesions on specific binding. Both destruction of dentate granule cells with colchicine and ablation of the ipsilateral entorhinal cortex markedly reduced radioligand binding to a quisqualate-sensitive site (GLU A), but only the entorhinal lesion significantly reduced binding to a site that is less sensitive to quisqualate (GLU B). These results suggest that GLU A binding sites are localized mainly on the dentate granule cells, whereas GLU B binding sites are localized, in part, on the perforant path fibers, but not on granule cells.