Multiparametric optical coherence tomography imaging of the inner retinal hemodynamic response to visual stimulation.
Multiparametric optical coherence tomography imaging of the inner retinal hemodynamic response to visual stimulation.
复制标题
视网膜内血流动力学对视觉刺激的反应的多参数光学相干断层扫描成像。
DOI：
10.1117/1.jbo.18.8.086010
复制
发表时间：
2013
影响因子：
3.5
通讯作者：
Srinivasan,VivekJ
中科院分区：
文献类型：
--
作者：
Radhakrishnan,Harsha;Srinivasan,VivekJ
由于功能性磁共振成像的普及，对神经元激活的血流动力学反应是脑中的一种充分研究的现象。视网膜代表了一个光学可访问的平台，用于研究在中枢神经系统中的椎板特异性神经血管耦合;然而，由于方法的限制，这一直是具有挑战性的日期。我们展示了使用多普勒光学相干断层扫描（OCT）和OCT血管造影术在大鼠视网膜内层成像视觉刺激诱发充血的技术。具有三维运动校正的体积成像、表面流计算以及将动态信号归一化为静态信号是减少由运动引起的虚假变化的技术。我们预计，视网膜功能性充血的OCT成像可能会产生可行的生物标志物的疾病，如糖尿病视网膜病变，其中神经血管单位可能受损。
The hemodynamic response to neuronal activation is a well-studied phenomenon in the brain, due to the prevalence of functional magnetic resonance imaging. The retina represents an optically accessible platform for studying lamina-specific neurovascular coupling in the central nervous system; however, due to methodological limitations, this has been challenging to date. We demonstrate techniques for the imaging of visual stimulus-evoked hyperemia in the rat inner retina using Doppler optical coherence tomography (OCT) and OCT angiography. Volumetric imaging with three-dimensional motion correction,en faceflow calculation, and normalization of dynamic signal to static signal are techniques that reduce spurious changes caused by motion. We anticipate that OCT imaging of retinal functional hyperemia may yield viable biomarkers in diseases, such as diabetic retinopathy, where the neurovascular unit may be impaired.