A wireless electrochemiluminescence detector applied to direct and indirect detection for electrophoresis on a microfabricated glass device
A wireless electrochemiluminescence detector applied to direct and indirect detection for electrophoresis on a microfabricated glass device
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DOI：
10.1021/ac0100300
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发表时间：
2001-07-15
影响因子：
7.4
通讯作者：
Manz, A
中科院分区：
文献类型：
--
作者：
Arora, A;Eijkel, JCT;Manz, A
提出了一种新型的电化学发光检测器。该检测器用于二氯三（2，2 '-联吡啶）钌（II）水合物[Ru-（bpy）]和二氯三（1，10-菲咯啉）钌（II）水合物[Ru（phen）]的胶束电动色谱分离。它由一个微加工的“U”形浮动铂电极横跨分离通道放置。U形的腿分别用作工作电极和反电极。ECL反应所需的电势差是：在电泳分离期间，通过分离通道中可用的电场在Pt电极处产生。初步实验表明，胶束电动分离和直接ECL检测10（-16）的Ru（phen）（10（-6）M）和4.5 × 10（-16）mol的Ru（bpy）（5 × 10（-6）M）。此外，初步结果显示了三种氨基酸的间接检测。检测位置的高电压不会干扰电化学。
A novel electrochemiluminescence (ECL) detector is presented in this article. The detector is applied for micellar electrokinetic chromatographic separation of dichlorotris(2,2'-bipyridyl)ruthenium(II) hydrate [Ru-(bpy)] and dichlorotris(1,10-phenanthroline)ruthenium-(II) hydrate [Ru(phen)] on a microfabricated glass device. It consists of a microfabricated "U"-shape floating platinum electrode placed across the separation channel. The legs of the U function respectively as working and counter electrode. The required potential difference for the ECL reaction is: generated at the Pt electrode by the electric field available in the separation channel during electrophoretic separation. Initial experiments demonstrate a micellar electrokinetic separation and direct ECL detection of 10(-16) of Ru(phen) (10(-6) M) and 4.5 x 10(-16) mol of Ru(bpy) (5 x 10(-6) M). Also, preliminary results show the indirect detection of three amino acids. The high voltage at the location of detection does not interfere with the electrochemistry.