Gold nanoparticle size controlled by polymeric Au(I) thiolate precursor size
Gold nanoparticle size controlled by polymeric Au(I) thiolate precursor size
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DOI：
10.1021/ja076333e
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发表时间：
2008-01-23
影响因子：
15
通讯作者：
Hainfeld, James F.
中科院分区：
文献类型：
--
作者：
Brinas, Raymond P.;Hu, Minghui;Hainfeld, James F.
我们开发了一种通过在还原前改变溶液的 pH 值（5.5 至 8.0 之间）来制备谷胱甘肽封端的尺寸可控的金纳米颗粒（Au NP，2-6 nm）的方法。该方法基于聚合纳米颗粒前体 Au(I)-谷胱甘肽聚合物的形成，其尺寸和密度根据 pH 值而变化。动态光散射、尺寸排阻色谱和紫外-可见光谱结果表明，较低的pH值有利于较大和较致密的聚合物前体，而较高的pH值有利于较小和较低密度的前体。因此，较大的前体导致较大的金纳米粒子的形成，而较小的前体导致较小的金纳米粒子的形成。采用这种策略，通过混合配体方法制备了用次氮基乙酸镍（II）（Ni-NTA）基团功能化的金纳米粒子。这些 Ni-NTA 功能化 Au NP 表现出与 6x-组氨酸标记的腺病毒血清型 12 旋钮蛋白的特异性结合，证明了它们在生物分子标记应用中的实用性。
We developed a method in preparing size-controllable gold nanoparticles (Au NPs, 2-6 nm) capped with glutathione by varying the pH (between 5.5 and 8.0) of the solution before reduction. This method is based on the formation of polymeric nanoparticle precursors, Au(I)-glutathione polymers, which change size and density depending on the pH. Dynamic light scattering, size exclusion chromatography, and UV-vis spectroscopy results suggest that lower pH values favor larger and denser polymeric precursors and higher pH values favor smaller and less dense precursors. Consequently, the larger precursors led to the formation of larger Au NPs, whereas smaller precursors led to the formation of smaller Au NPs. Using this strategy, Au NPs functionalized with nickel(II) nitriloacetate (Ni-NTA) group were prepared by a mixed-ligand approach. These Ni-NTA functionalized Au NPs exhibited specific binding to 6x-histidine-tagged Adenovirus serotype 12 knob proteins, demonstrating their utility in biomolecular labeling applications.