Differentiation of a cell line from olfactory epithelium is induced by dibutyryladenosine 3',5'-cyclic monophosphate and 12-O-tetradecanoylphorbol-13-acetate.
Differentiation of a cell line from olfactory epithelium is induced by dibutyryladenosine 3',5'-cyclic monophosphate and 12-O-tetradecanoylphorbol-13-acetate.
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由二丁酰基腺苷 3,5-环单磷酸和 12-O-十四烷酰佛波醇-13-乙酸酯诱导细胞系从嗅觉上皮分化。
DOI：
10.1016/0006-8993(86)90475-0
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Teeter,JH
中科院分区：
文献类型：
--
作者：
Goldstein,NI;Quinn,MR;Teeter,JH
嗅觉感受器神经元经历不断的替换，新细胞从位于嗅觉上皮中的一群基底细胞分化而来。我们曾报道从小鼠嗅觉上皮(MoE CL1)中分离出一株对两种分化促进剂--二丁基腺苷3‘，5’-环磷酸腺苷(db-cAMP)和12-O-十四酰佛波醇-13-乙酸酯(TPA)敏感的细胞系。在db-cAMP或TPA存在下生长的细胞在72-96h的滞后期后变为双极，具有长的突起样突起。在db-cAMP或TPA中培养至少一代的细胞，或在db-cAMP和TPA的混合物中培养的细胞在24 h内出现双极形态，db-cAMP、TPA或db-cAMP+TPA培养的细胞带有突起的细胞比例显著增加。在暴露于db-cAMP的培养物中，也观察到种群倍增时间的增加和饱和密度的降低。暴露于TPA的细胞继续以与对照组相同的速度分裂，而db-cAMP+TPA处理的细胞则经历了终末分化。外周型苯二氮卓类结合部位位于嗅觉上皮细胞的感受器神经元上，存在于未分化细胞的匀浆膜中。经db-cAMP+TPA作用7天的分化细胞显示，外周型苯二氮卓结合位点密度(Bmax)增加了65%。对照培养的未分化细胞在注入电流的过程中，膜电位或再生反应没有自发变化。然而，在形态分化的培养中，小的双极细胞能够产生几种类型的自发膜电位变化，包括缓慢、规则的振荡；不规则、缓慢和快速的去极化；以及一系列动作电位。
Olfactory receptor neurons undergo continual replacement with new cells differentiating from a population of basal cells located in the olfactory epithelium. We have previously described the isolation of a cell line from murine olfactory epithelium (MOE CL1) which is now shown to be sensitive to two differentiation promotion agents, dibutyryladenosine 3′, 5′-cyclic monophosphate (db-cAMP) and12-O-tetradecanoylphorbol-13-acetate(TPA). Cells grown in the presence of db-cAMP or TPA, after a lag phase of 72–96 h, became bipolar, with long neurite-like processes. Cells conditioned for at least one passage in db-cAMP or TPA, or cells exposed to a mixture of db-cAMP and TPA, attained a bipolar morphology within 24 h. Cultures grown with db-cAMP, TPA or db-cAMP + TPA showed significant increases in the percentage of process-bearing cells. An increase in population doubling time and decrease in saturation density were also observed in cultures exposed to db-cAMP. Cells exposed to TPA continued to divide at the same rate as control cells, while those treated with db-cAMP + TPA underwent terminal differentiation. Peripheral-type benzodiazepine binding sites, which within the olfactory epithelium are localized on the receptor neurons, were present in homogenate membranes of undifferentiated cells. Cells differentiated by exposure to db-cAMP + TPA for 7 days showed a 65% increase in the density (Bmax) of peripheral-type benzodiazepine binding sites. Undifferentiated cells in control cultures showed no spontaneous changes in membrane potential or regenerative responses during current injection. Small, bipolar cells in morphologically differentiated cultures, however, were capable of generating several types of spontaneous changes in membrane potential including slow, regular oscillations; irregular, slow and fast depolarizations; and trains of action potentials.