Contrasting Roles of the Apoplastic Aspartyl Protease APOPLASTIC, ENHANCED DISEASE SUSCEPTIBILITY1-DEPENDENT1 and LEGUME LECTIN-LIKE PROTEIN1 in Arabidopsis Systemic Acquired Resistance
Contrasting Roles of the Apoplastic Aspartyl Protease APOPLASTIC, ENHANCED DISEASE SUSCEPTIBILITY1-DEPENDENT1 and LEGUME LECTIN-LIKE PROTEIN1 in Arabidopsis Systemic Acquired Resistance
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DOI：
10.1104/pp.114.239665
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发表时间：
2014-06-01
期刊：
影响因子：
7.4
通讯作者：
Vlot, A. Corina
中科院分区：
文献类型：
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作者：
Breitenbach, Heiko H.;Wenig, Marion;Vlot, A. Corina
全身性获得的抗性（SAR）是一种可诱导的免疫反应，取决于疾病易感性增强1（EDS1）。在这里，我们表明拟南芥（拟南芥）EDS1在原发性感染的叶片中两种SAR信号产生和全身未感染组织中的SAR信号感知需要。与SAR信号的产生相反，响应于曲线病的假单胞菌伴随着效应蛋白AVRRPM1的假单胞菌，局部抗性仍然完好无损。我们利用EDS1突变体的SAR特异性表型来鉴定有条件表达AVRRPM1的植物中的新SAR调节蛋白。对表达AVRRPM1的野生型和EDS1突变植物的蛋白质组学分析对凋亡的富集提取物进行了鉴定，从而鉴定了12种质外塑料，EDS1依赖性（AED）蛋白。编码AED1的基因，预测的天冬氨酸蛋白酶和另一个AED AED豆科素凝集素样蛋白1（LLP1）在SAR信号期间局部和系统地诱导了局部和全身诱导，并通过水杨酸（SA）或其功能模拟局部诱导局部。 - 硫二唑-7-核糖酸S-甲基酯。由于AED1-羟谷丁蛋白的有条件过度积累会抑制SA诱导的耐药性和SAR，但没有局部抗性，因此数据表明AED1是调节全身免疫力的稳态反馈机制的一部分。在LLP1突变植物中，SAR受到损害，而通常与EDS1和SA相关的局部电阻以及对外源SA的反应似乎在很大程度上没有受到影响。总之，这些数据表明LLP1可能与SA并行促进系统性而不是局部免疫。我们的分析揭示了SAR的新阳性和负组成部分，并加强了SAR代表植物免疫的不同阶段，超出了局部抗性。
Systemic acquired resistance (SAR) is an inducible immune response that depends on ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1). Here, we show that Arabidopsis (Arabidopsis thaliana) EDS1 is required for both SAR signal generation in primary infected leaves and SAR signal perception in systemic uninfected tissues. In contrast to SAR signal generation, local resistance remains intact in eds1 mutant plants in response to Pseudomonas syringae delivering the effector protein AvrRpm1. We utilized the SAR-specific phenotype of the eds1 mutant to identify new SAR regulatory proteins in plants conditionally expressing AvrRpm1. Comparative proteomic analysis of apoplast-enriched extracts from AvrRpm1-expressing wild-type and eds1 mutant plants led to the identification of 12 APOPLASTIC, EDS1-DEPENDENT (AED) proteins. The genes encoding AED1, a predicted aspartyl protease, and another AED, LEGUME LECTIN-LIKE PROTEIN1 (LLP1), were induced locally and systemically during SAR signaling and locally by salicylic acid (SA) or its functional analog, benzo 1,2,3-thiadiazole-7-carbothioic acid S-methyl ester. Because conditional overaccumulation of AED1-hemagglutinin inhibited SA-induced resistance and SAR but not local resistance, the data suggest that AED1 is part of a homeostatic feedback mechanism regulating systemic immunity. In llp1 mutant plants, SAR was compromised, whereas the local resistance that is normally associated with EDS1 and SA as well as responses to exogenous SA appeared largely unaffected. Together, these data indicate that LLP1 promotes systemic rather than local immunity, possibly in parallel with SA. Our analysis reveals new positive and negative components of SAR and reinforces the notion that SAR represents a distinct phase of plant immunity beyond local resistance.