Following the Footsteps of Chlamydial Gene Regulation.
Following the Footsteps of Chlamydial Gene Regulation.
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DOI：
10.1093/molbev/msv193
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发表时间：
2015-12
影响因子：
10.7
通讯作者：
Horn M
中科院分区：
文献类型：
--
作者：
Domman D;Horn M
基因表达的调节确保生物体对刺激做出反应并经历适当的发育。虽然细菌中的调控网络已经在模式微生物中进行了研究，但对于这些网络在专性细胞内细菌中的进化和可塑性几乎一无所知。衣原体门包含大量与宿主相关的微生物，包括几种人类病原体。衣原体在专性细胞内细菌中是独特的，因为它们经历复杂的双相发育周期，其中大量基因受到时间调控。再加上转录因子的数量很少，这些生物体提供了一个模型来研究细胞内生物体中调控网络的进化。我们提供了第一个全面的分析，探索跨门的监管网络的多样性和演变。我们利用比较基因组学的方法来构建预测的共调控网络，揭示了属和家族特异性的调控基序和架构，最值得注意的是那些与毒力相关的基因。令人惊讶的是，我们的分析表明，很少有调控成分是保守的整个门，和那些保守的参与开发的细胞内生态位。因此，我们的研究有助于深入了解衣原体进化的一个组成部分，否则在很大程度上仍然未被探索。
Regulation of gene expression ensures an organism responds to stimuli and undergoes proper development. Although the regulatory networks in bacteria have been investigated in model microorganisms, nearly nothing is known about the evolution and plasticity of these networks in obligate, intracellular bacteria. The phylum Chlamydiae contains a vast array of host-associated microbes, including several human pathogens. The Chlamydiae are unique among obligate, intracellular bacteria as they undergo a complex biphasic developmental cycle in which large swaths of genes are temporally regulated. Coupled with the low number of transcription factors, these organisms offer a model to study the evolution of regulatory networks in intracellular organisms. We provide the first comprehensive analysis exploring the diversity and evolution of regulatory networks across the phylum. We utilized a comparative genomics approach to construct predicted coregulatory networks, which unveiled genus- and family-specific regulatory motifs and architectures, most notably those of virulence-associated genes. Surprisingly, our analysis suggests that few regulatory components are conserved across the phylum, and those that are conserved are involved in the exploitation of the intracellular niche. Our study thus lends insight into a component of chlamydial evolution that has otherwise remained largely unexplored.