The holding temperature of blood during a delay to processing can affect serum and plasma protein measurements.
The holding temperature of blood during a delay to processing can affect serum and plasma protein measurements.
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DOI：
10.1038/s41598-021-85052-5
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发表时间：
2021-03-22
影响因子：
4.6
通讯作者：
Costello E
中科院分区：
文献类型：
--
作者：
Ashworth M;Small B;Oldfield L;Evans A;Greenhalf W;Halloran C;Costello E
寻求准确的血液传播生物标志物用于诊断、预后和治疗分层。一致的血液处理对于有意义的数据解释至关重要，然而，处理过程中的延迟有时是不可避免的。我们研究了立即将血液样本置于冰上与室温下1 h（参考方案）以及在处理延迟3 h期间将样本置于冰上与室温下的影响。使用Luminex多重分析评估健康受试者中的细胞因子（n = 29）和糖尿病相关蛋白（n = 15），我们观察到将血液样品立即置于冰上降低了几种细胞因子的血清水平，包括派-1、MIP 1-β、IL-9、RANTES和IL-8。在处理延迟期间，一些分析物，例如瘦素和胰岛素，显示血清或血浆值几乎没有变化。然而，与参考方案相比，对于研究的大约一半分析物，无论放置温度如何，延迟都会改变测量水平。血清和血浆之间的影响不同，对于某些分析物，水平变化的方向因个体而异。延迟期间样品的最佳保持温度是分析物特异性的。总之，方案偏离可能导致血液分析物水平发生显著变化。在可能的情况下，应以分析物特定的方式预先确定血液处理方案。
Accurate blood-borne biomarkers are sought for diagnosis, prognosis and treatment stratification. Consistent handling of blood is essential for meaningful data interpretation, however, delays during processing are occasionally unavoidable. We investigated the effects of immediately placing blood samples on ice versus room temperature for 1 h (reference protocol), and holding samples on ice versus room temperature during a 3 h delay to processing. Using Luminex multi-plex assays to assess cytokines (n = 29) and diabetes-associated proteins (n = 15) in healthy subjects, we observed that placing blood samples immediately on ice decreased the serum levels of several cytokines, including PAI-1, MIP1-β, IL-9, RANTES and IL-8. During a delay to processing, some analytes, e.g. leptin and insulin, showed little change in serum or plasma values. However, for approximately half of the analytes studied, a delay, regardless of the holding temperature, altered the measured levels compared to the reference protocol. Effects differed between serum and plasma and for some analytes the direction of change in level varied across individuals. The optimal holding temperature for samples during a delay was analyte-specific. In conclusion, deviations from protocol can lead to significant changes in blood analyte levels. Where possible, protocols for blood handling should be pre-determined in an analyte-specific manner.
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影响因子：
4.4
作者：
Tuck MK;Chan DW;Chia D;Godwin AK;Grizzle WE;Krueger KE;Rom W;Sanda M;Sorbara L;Stass S;Wang W;Brenner DE
通讯作者：
Brenner DE
DOI：
10.1182/blood.v38.1.39.39
发表时间：
1971-01-01
期刊：
BLOOD-THE JOURNAL OF HEMATOLOGY
影响因子：
--
作者：
KATTLOVE, HE;ALEXANDER, B
通讯作者：
ALEXANDER, B
影响因子：
2.7
作者：
Reid, TJ;LaRussa, VF;Gorogias, M
通讯作者：
Gorogias, M
影响因子：
3.8
作者：
Henno, Linda Torrissen;Storjord, Min;Brekke, Ole-Lars
通讯作者：
Brekke, Ole-Lars
影响因子：
9.3
作者：
Timms, John F.;Arslan-Low, Elif;Menon, Usha
通讯作者：
Menon, Usha