Expression of nephrin by human pancreatic islet endothelial cells
Expression of nephrin by human pancreatic islet endothelial cells
复制标题
人胰岛内皮细胞表达去氧肾上腺素
作者：
M. Zanone;E. Favaro;S. Doublier;B. Lozanoska;M. Deregibus;J. Greening;G. Huang;N. Klein;P. Perin;M. Peakman;G. Camussi
目的/假设胰岛微循环具有类似于肾脏肾小球基因的转录的形态特征。在这项研究中，我们纯化并表征了从人类胰岛分离的微血管内皮细胞（MEC）提取肾素胰岛在这些细胞上的表达和分布。提取并使用抗CD105涂层的免疫磁珠纯化并纯化，通过表达经典的内皮标记物和基本的高水平表达，证实了其内皮特征。 - 可诱导的血管细胞粘附分子1。细胞裂解物以及RT-PCR上的分析和蛋白质印迹。在两种情况下，在两种情况下，在人类胰岛胰岛中，都在培养的胰岛MEC和人类胰岛中检测到培养的胰岛MEC的细胞肾上腺素。 TNF-α处理诱导了一个或多个肾素表达中蛋白质的显着还原和重新分布。与其他部位的蛋白质或微血管细胞中的蛋白质或mRNA水平无法检测到印迹和RT-PCR研究。胰岛MEC暴露了独特的形态特征，表明潜在病理生理重要性的功能专业化。
Aims/hypothesisThe islet microcirculation has morphological characteristics resembling those of renal glomeruli. Transcription of the nephrin gene, a highly specific barrier protein of the slit diaphragm of podocyte foot processes, has been reported in the pancreas, although its cellular localisation and function remain to be defined. In this study, we purified and characterised microvascular endothelial cells (MECs) isolated from human islets and investigated the expression and distribution of nephrin on these cells.MethodsHuman islet MECs were extracted and purified using anti-CD105-coated immunomagnetic beads and their endothelial characteristics were confirmed by expression of classical endothelial markers and basal high-level expression of intercellular adhesion molecule-1 and TNF-α-inducible vascular cell adhesion molecule-1. Nephrin expression was assessed by immunofluorescence, flow cytometric analysis and western blotting on cell lysates, as well as by RT-PCR.ResultsImmunofluorescence studies detected nephrin in a fine, punctate, diffuse pattern on cultured islet MECs, and also in human pancreatic islet sections. In both cases nephrin colocalised with endothelial markers. TNF-α treatment induced a marked reduction and redistribution of the protein in one or multiple aggregates. Nephrin expression was confirmed by flow cytometry, western blotting and RT-PCR studies. In contrast, nephrin could not be detected at the protein or mRNA level in human macro- and microvascular cells from other sites.Conclusions/interpretationNephrin is expressed at protein and mRNA levels in islet microendothelium, supporting the hypothesis that islet MECs exhibit distinctive morphological characteristics that indicate functional specialisation of potential pathophysiological importance.
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影响因子：
9.8
作者：
Lenkkeri, U;Männikkö, M;Tryggvason, K
通讯作者：
Tryggvason, K
影响因子：
19.6
作者：
Lahdenperä, J;Kilpeläinen, P;Tryggvason, K
通讯作者：
Tryggvason, K
胰岛移植微血管的组织发生和超微结构。
DOI：
--
发表时间：
1995
期刊：
The American journal of pathology.
影响因子：
--
作者：
Vajkoczy,P;Olofsson,AM;Lehr,HA;Leiderer,R;Hammersen,F;Arfors,KE;Menger,MD
通讯作者：
Menger,MD
影响因子：
7.7
作者：
Treutelaar, MK;Skidmore, JM;Burant, CF
通讯作者：
Burant, CF
影响因子：
3.5
作者：
Putaala, H;Soininen, R;Tryggvason, K
通讯作者：
Tryggvason, K