QUANTITATIVE STUDIES OF CHANGES IN CORTICAL GRANULE NUMBER AND DISTRIBUTION IN THE MOUSE OOCYTE DURING MEIOTIC MATURATION
QUANTITATIVE STUDIES OF CHANGES IN CORTICAL GRANULE NUMBER AND DISTRIBUTION IN THE MOUSE OOCYTE DURING MEIOTIC MATURATION
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DOI：
10.1016/0012-1606(88)90425-3
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发表时间：
1988-11-01
影响因子：
2.7
通讯作者：
RANGARAJAN, S
中科院分区：
文献类型：
--
作者：
DUCIBELLA, T;ANDERSON, E;RANGARAJAN, S
在小鼠卵母细胞减数分裂成熟过程中，皮质颗粒在卵母细胞皮质中的分布发生了很大的变化。为了确定它们在排卵时分布变化的机制，对CG密度、每个卵母细胞的总数和结构域面积进行了定量。通过透镜culinaris凝集素-生物素和德克萨斯红-链霉亲和素荧光以及电子显微镜在显微镜下观察CG。未成熟的生发泡期（GV）卵母细胞从成年小鼠有一个连续的皮质定位与一些内部颗粒。成熟的卵母细胞有一个不对称的皮质分布与CG-自由域，覆盖减数分裂II中期纺锤体，占据40%的皮质。成熟卵母细胞的颗粒占据皮层和GV卵母细胞的整个皮层的平均CG密度分别为43和34 CG/100 μ m2。CG/卵母细胞的平均总数为4127（成熟）和7440（GV），并且在具有两个原核的受精卵母细胞中不存在染色。钙离子载体（A23187）激活的成熟卵母细胞的平均总数为1235 CG，其中一些可能已经在胞吐的过程中。第一极体的CG很少，因此不太可能解释GV和成熟卵母细胞之间CG数量的差异。在成熟的小鼠卵母细胞中，CG的总数量较少，密度较高，这表明胞吐和再分配是CG-自由域发育的合理机制。受精前的胞吐作用可以解释精子穿透的位点，其他人已经报道发生在小鼠减数分裂纺锤体对面的半球。
Cortical granules (CGs) undergo a substantial change in distribution in the mouse oocyte cortex during meiotic maturation. In order to determine the mechanism of their change in distribution near the time of ovulation, CG density, total number per oocyte, and domain areas were quantitated. CGs were visualized microscopically by Lens culinaris agglutinin-biotin and Texas red-strepavidin fluorescence as well as by electron microscopy. Immature germinal vesicle stage (GV) oocytes from adult mice had a continuous cortical localization with some interior granules. Mature oocytes had an asymmetric cortical distribution with a CG-free domain, overlying the meiosis II metaphase spindle, occupying 40% of the cortex. The mean CG densities of the granule-occupied cortex of mature oocytes and the entire cortex of GV oocytes were 43 and 34 CGs/100 .mu.m2, respectively. The mean total numbers of CGs/oocyte were 4127 (mature) and 7440 (GV), and staining was absent in fertilized oocytes with two pronuclei. Calcium ionophore (A23187)- activated mature oocytes had a mean total number of 1235 CGs, some of which may have been in the process of exocytosis. The first polar body had few CGs, and thus was unlikely to account for the difference in CG number between GV and mature oocytes. The smaller total number and higher density of CGs in mature mouse oocytes suggests that both exocytosis and redistribution are plausible mechanisms for the development of the CG-free domain. Prefertilization exocytosis could account for the locus of sperm penetration which others have reported to occur in the hemisphere opposite the meiotic spindle in the mouse.