Designing PCR primers painlessly.
Designing PCR primers painlessly.
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DOI：
10.1128/jmbe.v15i1.634
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发表时间：
2014-05
影响因子：
1.9
通讯作者：
Lopilato J
中科院分区：
文献类型：
--
作者：
Feeney M;Murphy K;Lopilato J
学生使用基于网络的工具设计引物，沿着原核基因的打印序列，显示DNA链和编码链翻译成氨基酸。 每个引物的解链温度被确定，并且在温度上最接近的一对引物可以用于扩增基因并将其克隆到载体中，用于一学期的实验室练习。通过这个引物设计练习，学生们可以直接看到DNA扩增过程中所涉及的内容。
Students design primers using web-based tools along with a printed sequence of a prokaryotic gene showing both DNA strands and translation of the coding strand into amino acids. The melting temperature of each primer is determined and the pair closest in temperature can be used to amplify and clone a gene into a vector for a one-semester laboratory exercise. By working through this primer design exercise, students see first hand what is involved in the process of amplifying DNA.