Experimental Study of Ectopic Osteogenesis of Bone Marrow Mesenchymal Stem Cells Induced by KLD-12 Polypeptide/rhBMP-2/BMSC Composite Fiber Gels
Experimental Study of Ectopic Osteogenesis of Bone Marrow Mesenchymal Stem Cells Induced by KLD-12 Polypeptide/rhBMP-2/BMSC Composite Fiber Gels
复制标题
KLD-12多肽/rhBMP-2/BMSC复合纤维凝胶诱导骨髓间充质干细胞异位成骨的实验研究
DOI：
10.1166/jbt.2019.1990
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发表时间：
2019-03
影响因子：
0.1
通讯作者：
Sun Jianhua
中科院分区：
文献类型：
--
作者：
Wang Bingxuan;Du Yan;Li Xiangchen;Sun Jianhua
目的观察KLD-12多肽/重组人骨形态发生蛋白2（rhBMP-2）纤维凝胶复合支架与兔骨髓间充质干细胞（BMSCs）复合植入兔臀肌内的成骨效果。将各移植物（实验组A：KLD-12多肽/rhBMP-2/BMSC凝胶;阳性对照组B：KLD-12多肽/rhBMP-2凝胶;阳性对照组C：rhBMP-2;阴性对照组D：KLD-12凝胶）分别植入臀肌肌袋内。分别于术后4、8、12周处死动物，进行X线片、大体观察、显微CT、组织形态学及碱性磷酸酶（ALP）活性测定。结果X线片、大体观察及显微CT结果显示，A、B、C三组中A组成骨最明显，D组无明显成骨。HE染色、Giemsa染色及ALP活性检测结果显示，A、B、C组均能形成成熟骨组织，其中A组成熟骨组织形成最明显。位于KLD-12多肽凝胶内的BMSCs和rhBMP-2发挥协同成骨作用。
ObjectiveTo investigate the osteogenic effect of KLD-12 polypeptide/recombinant human bone morphogenetic protein 2 (rhBMP-2) fiber gel composite scaffolds seeded with rabbit bone marrow mesenchymal stem cells (BMSCs) that were implanted in rabbit gluteal muscle.MethodsBMSCs were isolated and cultured by a density gradient method, and a muscle bag model was established in the gluteal muscle of rabbits. Each graft (experimental group A: KLD-12 polypeptide/rhBMP-2/BMSC gel; positive control group B: KLD-12 polypeptide/rhBMP-2 gel; positive control group C: rhBMP-2; negative control group D: KLD-12 gel) was separately implanted into the muscle pocket of the gluteal muscle. The rabbits were sacrificed at 4, 8, and 12 weeks postoperation for X-ray analysis, gross observation, micro-CT analysis, histomorphological staining, and an alkaline phosphatase (ALP) activity assay.ResultsX-ray, gross observation and micro-CT results showed that osteogenesis among groups A, B and C was the most obvious in group A, while group D showed no obvious osteogenesis. Hematoxylin-eosin (HE) staining, Giemsa staining and ALP activity results showed that mature bone tissue was formed in groups A, B and C and that mature bone tissue formation was the most obvious in group A.ConclusionThe KLD-12 polypeptide/rhBMP-2 gel seeded with BMSCs showed good osteogenesis in the gluteal muscle. BMSCs and rhBMP-2, which were located within the KLD-12 polypeptide gel, exerted a synergistic osteogenic effect.