Changes of mitochondrial ultrastructure and function during ageing in mice and Drosophila
Changes of mitochondrial ultrastructure and function during ageing in mice and Drosophila
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DOI：
10.7554/elife.24662
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发表时间：
2017-07-12
期刊：
影响因子：
7.7
通讯作者：
Kuehlbrandt, Werner
中科院分区：
文献类型：
--
作者：
Brandt, Tobias;Mourier, Arnaud;Kuehlbrandt, Werner
衰老是内在生理功能的逐渐衰退。我们通过电子冷冻断层扫描和呼吸仪研究了衰老对小鼠和果蝇线粒体超微结构和功能的影响。我们在两种模式生物中发现了明显的与年龄相关的变化。小鼠心脏的线粒体功能和超微结构得以维持，而小鼠肝脏线粒体亚群的膜形态则表现出与年龄相关的变化。来自年轻和年老小鼠肾脏的线粒体亚群类似于细胞凋亡。在年老的果蝇中，呼吸活动受到损害，过氧化自由基的产生增加。在大约50%的老年果蝇线粒体中，内膜组织被破坏。这在内膜结构和功能衰退之间建立了明确的联系。线粒体受衰老影响的程度非常不同，这取决于生物体，也可能取决于同一生物体内的组织免受线粒体损伤的保护程度。
Ageing is a progressive decline of intrinsic physiological functions. We examined the impact of ageing on the ultrastructure and function of mitochondria in mouse and fruit flies (Drosophila melanogaster) by electron cryo-tomography and respirometry. We discovered distinct age-related changes in both model organisms. Mitochondrial function and ultrastructure are maintained in mouse heart, whereas subpopulations of mitochondria from mouse liver show age-related changes in membrane morphology. Subpopulations of mitochondria from young and old mouse kidney resemble those described for apoptosis. In aged flies, respiratory activity is compromised and the production of peroxide radicals is increased. In about 50% of mitochondria from old flies, the inner membrane organization breaks down. This establishes a clear link between inner membrane architecture and functional decline. Mitochondria were affected by ageing to very different extents, depending on the organism and possibly on the degree to which tissues within the same organism are protected against mitochondrial damage.