A close relationship between androgen levels and eumelanogenesis in the teleost red seabream (Pagrus major): Quantitative analysis of its seasonal variation and effects of oral treatment with methyl-testosterone
A close relationship between androgen levels and eumelanogenesis in the teleost red seabream (Pagrus major): Quantitative analysis of its seasonal variation and effects of oral treatment with methyl-testosterone
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DOI：
10.1016/j.cbpa.2010.01.021
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发表时间：
2010-06-01
影响因子：
2.3
通讯作者：
Kato, Keitaro
中科院分区：
文献类型：
--
作者：
Adachi, Kohsuke;Wakamatsu, Kazumasa;Kato, Keitaro
研究了真鲷（Pagrus major）雄性和雌性真黑素（eumelanin）、褐黑素（pheomelanin）、11-酮睾酮（11 KT）、亮度（L* 值）和性腺体指数（GSI：性腺质量/体质量× 100）的季节变化。在雄性中，吡咯-2，3，5-三羧酸（真黑素的标志物），11 KT和GSI的水平从9月开始急剧增加，并在3月和4月达到稳定水平，此时鱼类性成熟。这些结果与它们体色的亮度一致。使用来自男性的数据，观察到这四个变量（PTCA、11 CT、亮度和GSI）的所有组合具有高度相关性。在女性中，除GSI外，这些变量几乎没有变化。还分析了4-氨基-3-羟基苯丙氨酸（褐黑素的标志物），但其始终低于检测限。口服合成雄激素甲基睾丸酮2个月的幼红鲷诱导真黑素积累约3倍高于对照组。这些数据表明，硬骨鱼类中雄激素水平与真黑素积累之间存在密切关系。这是第一份雄激素以剂量依赖性方式影响黑色素积累的报告。(C)2010年爱思唯尔公司All rights reserved.
Male and female teleost seabream (Pagrus major) were examined for seasonal variation of eumelanin, pheomelanin, 11-ketotestosterone (11KT, fish androgen), lightness (L* value) and Gonad Somatic index (GSI: gonad mass/body mass x 100). In males, levels of pyrrole-2,3,5-tricarboxylic acid (a marker of eumelanin), 11KT and the GSI increased sharply from September and plateaued in March and April when the fish are sexually mature. These results are consistent with the lightness of their body color. Using the data from males, a high correlation was observed for all combinations of those four variables (PTCA, 11CT, lightness and GSI). In females, little change was observed in those variables except for the GSI. 4-Amino-3-hydroxyphenylalanine (a marker of pheomelanin) was also analyzed, but it was below the detection limit at all times. Oral treatment of juvenile red seabream with synthetic androgen methyl-testosterone for 2 months induced eumelanin accumulation about 3 times higher than the control. These data show that there is a close relationship between androgen levels and eumelanin accumulation in teleosts. This is the first report that androgen affects melanin accumulation in a dose-dependent manner. (C) 2010 Elsevier Inc. All rights reserved.