The Abbott IMx automated benchtop immunochemistry analyzer system.
The Abbott IMx automated benchtop immunochemistry analyzer system.
复制标题
Abbott IMx 自动化台式免疫化学分析仪系统。
作者：
M. Fiore;J. Mitchell;T. Doan;Randy J. Nelson;Gary E. Winter;Cass J. Grandone;K. Zeng;R. Haraden;Jane B. Smith;K. Harris
我们描述了一种新的临床实验室仪器，IMx，用于自动化免疫检测在临床实验室。IMx采用了一种称为微粒捕获酶免疫测定（MEIA）的新技术，用于高分子量分析物的测定，以及用于半抗原测定的荧光偏振免疫测定（FPIA）。前表面荧光计用于以与MEIA中分析物的浓度成比例的速率定量荧光产物的酶促生成，并且荧光偏振光学系统用于定量FPIA中的结果。基于微处理器的仪器使用具有两个自由度的机械臂和旋转转盘来处理用于分析的样品。一个分析可以在30至40分钟内完成24名患者的标本与“步行”自动化。校准曲线可稳定至少两周。仪器控制包括软件标记的“命令键”、数字键盘和交互式显示器。结果输出到热敏打印机或计算机接口。
We describe a new clinical laboratory instrument, the IMx, used to automate immunoassay testing in the clinical laboratory. The IMx incorporates a novel technology called Microparticle capture Enzyme ImmunoAssay (MEIA) for assays of high-molecular-mass analytes, and fluorescence polarization immunoassay (FPIA) for hapten assays. A front-surface fluorometer is used to quantify the enzymatic generation of fluorescent product at a rate proportional to the concentration of the analyte in an MEIA, and a fluorescence polarization optical system is used to quantify results in an FPIA. The microprocessor-based instrument uses a robotic arm with two degrees of freedom and a rotating carousel to process the samples for assay. One assay can be done on each of 24 patients' specimens in 30 to 40 min with "walk-away" automation. Calibration curves are stable for at least two weeks. Instrument control involves software-labeled "command keys," a numeric keypad, and an interactive display. Results are output to a thermal printer or computer interface.