Human Placenta-Derived Multipotent Mesenchymal Stromal Cells Involved in Placental Angiogenesis via the PDGF-BB and STAT3 Pathways
Human Placenta-Derived Multipotent Mesenchymal Stromal Cells Involved in Placental Angiogenesis via the PDGF-BB and STAT3 Pathways
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DOI：
10.1095/biolreprod.115.131250
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发表时间：
2015-10-01
影响因子：
3.6
通讯作者：
Chen, Chie-Pein
中科院分区：
文献类型：
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作者：
Chen, Cheng-Yi;Liu, Shu-Hsiang;Chen, Chie-Pein
我们研究了人胎盘多能间充质基质细胞（hPMSCs）的平滑肌细胞分化能力，并确定了内皮细胞如何招募参与血管形成的hPMSCs。来自足月胎盘的hPMSC在诱导条件和不同基质基质下被诱导分化为平滑肌细胞。我们评估了来自脐静脉的内皮细胞的血小板衍生生长因子（PDGF）-BB表达并诱导hPMSC PDGFR-β和STAT 3活化。将内皮细胞与hPMSC共培养用于体外血管生成。然后通过小鼠胎盘移植试验进一步评估细胞分化能力。hPMSC可以分化成平滑肌细胞; I型和IV型胶原蛋白或层粘连蛋白支持这种分化。内皮细胞表达显著水平的PDGF-BB和激活的STAT 3转录活性的hPMSCs。内皮细胞条件培养液可诱导hPMSC迁移，STAT 3小干扰RNA转染或PDGFR-β阻断抗体可抑制hPMSC迁移，而PDGFR-α阻断抗体或同种型免疫球蛋白G（IgG; P
We studied the smooth muscle cell differentiation capability of human placental multipotent mesenchymal stromal cells (hPMSCs) and identified how endothelial cells recruit hPMSCs participating in vessel formation. hPMSCs from term placentas were induced to differentiate into smooth muscle cells under induction conditions and different matrix substrates. We assessed endothelial cells from umbilical veins for platelet-derived growth factor (PDGF)-BB expression and to induce hPMSC PDGFR-beta and STAT3 activation. Endothelial cells were co-cultured with hPMSCs for in vitro angiogenesis. Cell differentiation ability was then further assessed by mouse placenta transplantation assay. hPMSCs can differentiate into smooth muscle cells; collagen type I and IV or laminin support this differentiation. Endothelial cells expressed significant levels of PDGF-BB and activated STAT3 transcriptional activity in hPMSCs. Endothelial cell-conditioned medium induced hPMSC migration, which was inhibited by STAT3 small interfering RNA transfection or by pretreatement with PDGFR-beta-blocking antibody but not by PDGFR-alpha-blocking antibody or isotype immunoglobulin G (IgG; P