Effects of cholera toxin and actinomycin on synthesis of [35s]methionine-labeled proteins during progesterone-induced maturation of Xenopus laevis oocytes.
Effects of cholera toxin and actinomycin on synthesis of [35s]methionine-labeled proteins during progesterone-induced maturation of Xenopus laevis oocytes.
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霍乱毒素和放线菌素对黄体酮诱导非洲爪蟾卵母细胞成熟过程中[35s]甲硫氨酸标记蛋白合成的影响。
DOI：
10.1016/0012-1606(82)90005-7
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发表时间：
1982
影响因子：
2.7
通讯作者：
E. Baulieu
中科院分区：
文献类型：
--
作者：
E. Younglai;F. Godeau;B. Mulvihill;E. Baulieu
用双向聚丙烯酰胺凝胶电泳法研究了黄体酮诱导非洲爪哇卵母细胞减数分裂成熟过程中[35S]蛋氨酸掺入多肽的情况。持续观察到五种修饰：两个分子量约为150K道尔顿和pI5的多肽是新的蛋白质，两个代表掺入增加，一个代表掺入减少。霍乱毒素抑制孕酮诱导的修饰的出现。放线菌素和去核不能显著改变修饰。这些数据表明，孕酮诱导的蛋白质合成的改变与减数分裂细胞分裂的恢复之间存在很好的相关性。
The incorporation of [35S]methionine into polypeptides during progesterone-induced meiotic maturation ofXenopus laevisoocytes was studied by two-dimensional polyacrylamide gel electrophoresis. Five modifications were consistently observed: two polypeptides of an approximate molecular weight of 150K daltons and pI5 were new proteins, two represent increased incorporation and one was decreased incorporation. Cholera toxin inhibited the appearance of the modifications induced by progesterone. Actinomycin and enucleation did not significantly alter the modifications. These data indicate that a good correlation exists between the modifications in protein synthesis induced by progesterone and the resumption of meiotic cell division.