Quantification of mcrA by quantitative fluorescent PCR in sediments from methane seep of the Nankai Trough
Quantification of mcrA by quantitative fluorescent PCR in sediments from methane seep of the Nankai Trough
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DOI：
10.1111/j.1574-6941.2006.00101.x
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发表时间：
2006-07-01
影响因子：
4.2
通讯作者：
Horikoshi, Koki
中科院分区：
文献类型：
--
作者：
Nunoura, Takuro;Oida, Hanako;Horikoshi, Koki
建立了甲基辅酶M还原酶亚基A基因（mcrA）的荧光定量PCR方法，并应用于南开海槽增生棱镜缺氧甲烷渗漏沉积物中微生物群落的特征分析。所有先前鉴定的厌氧甲烷化菌（ANME） mcrA基因亚群都在海底以下25厘米的岩心中检测到，但发现mcrA基因的分布模式因深度而异。这些发现表明，在缺氧沉积物中，调节甲烷氧化的甲烷营养古菌具有明显的系统发育和生理多样性。该定量方法将为今后海洋缺氧沉积物中甲烷营养微生物生态系统的研究奠定基础。
A quantitative fluorogenic PCR method for group-specific methyl coenzyme M reductase subunit A genes (mcrA) from methanotrophic archaea was established and applied to the characterization of microbial communities in anoxic methane seep sediments at the accretionary prism of the Nankai Trough. All of the previously identified subgroups of anaerobic methanotroph (ANME) mcrA genes were detected in the cores up to 25 cm below the seafloor, but distributional patterns of mcrA genes were found to differ according to depth. These findings suggest a distinct distribution of phylogenetically and physiologically diverse methanotrophic archaea that mediate methane oxidation in the anoxic sediments. This quantification method will contribute to future investigations of methanotrophic microbial ecosystems in anoxic marine sediments.